Recombinant Human IL-17C (CHO-expressed) Protein

Catalog #: 9640-IL Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
9640-IL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
9640-IL-025

Recombinant Human IL-17C (CHO-expressed) Protein Bioactivity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17C (CHO-expressed) Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑17 RE Fc Chimera (Catalog # 8358-MR) is immobilize at 0.06 μg/mL, 100 μL/well, it binds Recombinant Human IL‑17C. The concentration of Recombinant Human IL‑17C that produces 50% of the optimal binding response is 0.2‑1.2 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-17C protein
His19-Val197

Accession #

Q9P0M4

N-terminal Sequence
Analysis

His19

Predicted Molecular Mass

20 kDa

SDS-PAGE

19-25 kDa, reducing conditions

Product Datasheets

9640-IL (with carrier)

Product Datasheet

COA

SDS

9640-IL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9640-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9640-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human IL-17 RE Chimera (Catalog # 8358-MR) is immobilize at 0.06 µg/mL, Recombinant Human IL-17C (Catalog # 9640-IL) binds with an ED₅₀ of 0.2-1.2 ng/mL.

Reconstitution Calculator

Background: IL-17C

Interleukin-17C (IL-17C) is a 15-20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17C shares 79% and 76% amino acid sequence identity with mouse and rat IL-17C, respectively (3). IL-17C binds to IL-17 RE with high affinity and to IL-17 RA with low affinity (4, 5). These two receptor chains can associate into a heterodimeric receptor for IL-17C (4-6). IL-17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and g/d T cells, while IL-17 RA is widely expressed (4, 5). IL-17 RE is required for mediating the pro-inflammatory and homeostatic actions of IL-17C in the skin and mucosa (1, 2). IL-17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4⁺ T cells, macrophages, and dendritic cells (4, 6-9). It is up-regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL-17 RE is reciprocally down-regulated in psoriatic lesions (10). The interaction of IL-17C with IL-17 RE promotes mucosal immunity through the induction of anti-bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL-17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL-17C is additionally upregulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL-17A, IL-17F, IL-22, CCR6, and CCL20 (5).

References