Recombinant Human IL-17C Protein

Name: Recombinant Human IL-17C Protein
Brand: R&D Systems
SKU: 1234-IL
Rating: 5 (1 reviews)

Catalog #: 1234-IL
4 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1234-IL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1234-IL-025

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Product Details

Citations (4)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17C Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17 RE Fc Chimera (Catalog # 8358-MR) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human IL-17C that produces 50% of the optimal binding response is 1.5-7.5 ng/mL.

Source

E. coli-derived human IL-17C protein
His19-Val197, with an N-terminal Met

Accession #

Q9P0M4.1

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

19.8 kDa (monomer)

Product Datasheets

1234-IL (with carrier)

Product Datasheet

COA

SDS

1234-IL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1234-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1234-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-17C

Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17C shares 79% and 76% amino acid sequence identity with mouse and rat IL‑17C, respectively (3). IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity (4, 5). These two receptor chains can associate into a heterodimeric receptor for IL‑17C (4‑6). IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma /δ T cells, while IL‑17 RA is widely expressed (4, 5). IL‑17 RE is required for mediating the pro‑inflammatory and homeostatic actions of IL‑17C in the skin and mucosa (1, 2). IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4⁺ T cells, macrophages, and dendritic cells (4, 6‑9). It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL‑17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20 (5).

References

Pappu, R. et al. (2012) Trends Immunol. 33:343.
Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
Chang, S.H. et al. (2011) Immunity 35:611.
Song, X. et al. (2011) Nat. Immunol. 12:1151.
Pfeifer, P. et al. (2012) Am. J. Respir. Cell Mol. Biol. 48:415.
Johnston, A. et al. (2013) J. Immunol. 190:2252.
Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.

Long Name

Interleukin 17C

Entrez Gene IDs

27189 (Human); 234836 (Mouse)

Alternate Names

CX2; Cytokine CX2; IL17C; IL-17C; IL-17CMGC126884; interleukin 17C; interleukin-17C; MGC138401

Related Research Areas

Citations for Recombinant Human IL-17C Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury
Authors: G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman
Cell Reports, 2022-12-20;41(12):111848.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
IL-17C-mediated innate inflammation decreases the response to PD-1 blockade in a model of Kras-driven lung cancer
Authors: F Ritzmann, C Jungnickel, G Vella, A Kamyschnik, C Herr, D Li, MM Menger, A Angenendt, M Hoth, A Lis, R Bals, C Beisswenge
Sci Rep, 2019-07-17;9(1):10353.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
Keratinocyte overexpression of IL-17C promotes psoriasiform skin inflammation.
Authors: Johnston A, Fritz Y, Dawes S, Diaconu D, Al-Attar P, Guzman A, Chen C, Fu W, Gudjonsson J, McCormick T, Ward N
J Immunol, 2013-01-28;190(5):2252-62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay