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Recombinant Human IL-21R Fc Chimera Protein, CF

Catalog #: 991-R2
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
991-R2-100
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Product Details
Citations (15)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-21R Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 40-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-21R protein
Human IL-21 R Subunit
(Cys20-Pro236)
Accession # Q9HBE5		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q9HBE5
N-terminal Sequence
Analysis
Cys20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.7 kDa (monomer)
SDS-PAGE
80-85 kDa, reducing conditions

Product Datasheets

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991-R2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

991-R2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 40-400 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21R

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common  gamma  chain ( gamma c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8+ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).

References
  1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
  2. Asao, H. et al. (2001) J. Immunol. 167:1.
  3. Habib, T. et al. (2002) Biochemistry 41:8725.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Ozaki, K. et al. (2002) Science 298:1630.
  7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  8. King, I.L. et al. (2010) J. Immunol. 185:6138.
  9. Kasaian, M.T. et al. (2002) Immunity 16:559.
  10. Frohlich, A. et al. (2009 Science 324:1576.
  11. Tortola, L. et al. (2010) Blood 116:5200.
  12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
  13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.
Long Name
Interleukin 21 Receptor
Entrez Gene IDs
50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names
CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

Citations for Recombinant Human IL-21R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Altered Circulating Follicular T Helper Cell Subsets and Follicular T Regulatory Cells Are Indicators of a Derailed B Cell Response in Lupus, Which Could Be Modified by Targeting IL-21R
    Authors: K Szabó, I Jámbor, K Pázmándi, N Nagy, G Papp, T Tarr
    International Journal of Molecular Sciences, 2022-10-13;23(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. DOCK8 is essential for LFA-1 dependent positioning of T follicular helper cells in germinal centers
    Authors: E Janssen, M Tohme, J Butts, SS Giguere, PT Sage, FE Velázquez, C Kam, E Milin, M Das, A Sobh, S Al-Tamemi, FW Luscinskas, F Batista, RS Geha
    JCI Insight, 2020-08-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Activation and induction of antigen-specific T follicular helper cells (TFH) play a critical role in LAIV-induced human mucosal anti-influenza antibody response
    Authors: A Aljurayyan, S Puksuriwon, M Ahmed, R Sharma, M Krishnan, S Sood, K Davies, D Rajashekar, S Leong, PS McNamara, S Gordon, Q Zhang
    J. Virol., 2018-05-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Allogeneic dendritic cells stimulated with antibodies against HLA class II polarize naive T cells in a follicular helper phenotype
    Authors: O Désy, S Béland, P Vallin, J Riopel, E Latulippe, E Wagner, SA De Serres
    Sci Rep, 2018-03-05;8(1):4025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. CD4 T-cell cytokines synergize to induce proliferation of malignant and nonmalignant innate intraepithelial lymphocytes
    Authors: YM Kooy-Winke, D Bouwer, GM Janssen, A Thompson, MH Brugman, F Schmitz, AH de Ru, T van Gils, G Bouma, JJ van Rood, PA van Veelen, ML Mearin, CJ Mulder, F Koning, J van Bergen
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-03;0(0):.
    Applications: Bioassay
  6. Augmentation of Circulating Follicular Helper T Cells and Their Impact on Autoreactive B Cells in Myasthenia Gravis
    J Immunol, 2016-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis.
    Authors: Xing R, Yang L, Jin Y, Sun L, Li C, Li Z, Zhao J, Liu X
    Scand J Immunol, 2016-01-01;83(1):64-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Cell-Intrinsic gp130 Signaling on CD4+ T Cells Shapes Long-Lasting Antiviral Immunity.
    Authors: Harker J, Wong K, Dolgoter A, Zuniga E
    J Immunol, 2015-06-17;195(3):1071-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  9. CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
    Authors: Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe J, Harter G, Gruner B, Fabricius D, Lotfi R, Schwarz K, Schutz C, Honig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdorfer B
    J Immunol, 2015-03-16;194(8):3768-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Impaired antibody response to influenza vaccine in HIV-infected and uninfected aging women is associated with immune activation and inflammation.
    Authors: Parmigiani A, Alcaide M, Freguja R, Pallikkuth S, Frasca D, Fischl M, Pahwa S
    PLoS ONE, 2013-11-13;8(11):e79816.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013-02-10;131(4):1146-56, 1156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
    Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
    J. Immunol., 2010-02-05;184(5):2337-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009-03-02;119(10):1424-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009-01-05;113(13):2955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006-05-08;55(12):1774-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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