Recombinant Human IL-26/AK155 Dimer Protein Summary
Product Specifications
Lys22-Gln171, with an N-terminal Met
Analysis
Disulfide-linked homodimer
36 kDa, non-reducing conditions
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1870-IL
| Formulation | Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol with BSA as a carrier protein. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1870-IL/CF
| Formulation | Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-26/AK155
IL-26 was originally cloned from herpesvirus saimiri (HVS)-transformed T-cells and named AK155 (1). It is a member of the IL-10 family of class II cytokines that signal via heterodimeric receptor complexes composed of two type I transmembrane receptor subunits (2). The human IL-26 gene has been mapped to chromosome 12q15. It encodes a 171 amino acid polypeptide with a 21 amino acid signal peptide. In addition to HVS-transformed T cells, IL-26 is also expressed in other virus transformed T cell lines, fresh peripheral mononuclear cells, activated NK cells and T cells. A mouse homologue of human IL-26 has not been identified. IL-26 binds with high-affinity to the heterodimeric complex consisting of the ligand-binding IL-20 R alpha and non ligand-binding IL-10 R beta (3). Activation of the receptor complex results in rapid phosphorylation of STAT1 and STAT3. Although the IL-26 receptor complex is highly specific for IL-26 and is not activated by other class II cytokines, the individual subunits of the IL-26 receptor complex are components in receptor complexes for other class II cytokines (1). IL-20 R alpha can form dimers with IL‑20 R beta to function as signaling receptors for IL-19, IL-20, and IL-24. IL-10 R beta can complex with IL-10 R alpha, IL-22 R, and IL-28 R alpha to transduce signals for IL-10, IL-22, and the three novel IFNs (IL-28A, IL-28B and IL-29), respectively. The physiological functions of IL-26 remain to be determined.
IL-26 was reported to be a homodimer in solution (1). E. coli-derived IL-26 produced by R&D Systems contains disulfide-linked homodimers and monomers. Both the pure monomeric and dimeric IL-26 can bind directly to IL-20 R alpha and induce STAT activation in COLO-205 cells (4). Besides IL-20 R alpha, R&D Systems IL-26 preparations have also been shown to bind IL‑20 R beta. The significance of this receptor-ligand interaction has not been investigated.
- Knappe. A. et al. (2000) J. Virology 74:3881.
- Renauld, J.-C. (2003) Nature Reviews Immunology 3:667.
- Sheikh, F. et al. (2004) J. Immunol. 172:2006.
- Unpublished data (2003) R&D Systems, Inc.
Citations for Recombinant Human IL-26/AK155 Dimer Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells
Authors: T Itoh, R Hatano, Y Horimoto, T Yamada, D Song, H Otsuka, Y Shirakawa, S Mastuoka, N Iwao, TM Aune, NH Dang, Y Kaneko, K Okumura, C Morimoto, K Ohnuma
Cell Death & Disease, 2021-05-21;12(6):520.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-26, a non-canonical mediator of DNA inflammatory stimulation, promotes TNBC engraftment and progression in association with neutrophils
Authors: TN Trotter, CW Shuptrine, LC Tsao, RD Marek, C Acharya, JP Wei, XY Yang, G Lei, T Wang, HK Lyerly, ZC Hartman
Cancer Res., 2020-05-04;0(0):.
Species: Human
Sample Types:
Applications: Cell Culture -
Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
PLoS ONE, 2017-12-05;12(12):e0188909.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
PLoS Biol, 2012-09-25;10(9):e1001395.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2.
Authors: Sheikh F, Baurin VV, Lewis-Antes A, Shah NK, Smirnov SV, Anantha S, Dickensheets H, Dumoutier L, Renauld JC, Zdanov A, Donnelly RP, Kotenko SV
J. Immunol., 2004-02-15;172(4):2006-10.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay
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