Recombinant Human IL-37/IL-1F7 Protein

Catalog #: 9225-IL
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
9225-IL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
9225-IL-025

Recombinant Human IL-37/IL-1F7 Protein Bioactivity

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Product Details

Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-37/IL-1F7 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells. The ED₅₀ for this effect is 0.25-1.25 μg/mL.

Source

E. coli-derived human IL-37/IL-1F7 protein
Ser2-Asp192

Accession #

Q9NZH6-2

N-terminal Sequence
Analysis

Ser2

Predicted Molecular Mass

21 kDa

SDS-PAGE

22 kDa, reducing conditions

Product Datasheets

9225-IL (with carrier)

Product Datasheet

COA

SDS

9225-IL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9225-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9225-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IL-37/IL-1F7 (Catalog # 9225-IL) inhibits LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells. The ED₅₀ for this effect is 0.25-1.25 μg/mL.

Reconstitution Calculator

Background: IL-37/IL-1F7

Human Interleukin-1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family (1-3). Alternative splicing generates multiple isoforms (IL-37a through e) with deletions in the N-terminal region of the molecule (4-7). IL-37b shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta, and IL-1ra, respectively. Mouse IL-37 has not been reported, but human IL-37b is active on mouse cells. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide. IL-37b is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (8). Experimental over-expression of IL-37b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (8, 9). Both unprocessed and mature IL-37b can form homodimers in solution (8, 10). Although IL-37b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (7, 10, 11). Alternatively, IL-37b will also bind to IL-18 BP, generating a complex that interacts with IL-18 R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (11). IL-37b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-37b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (12).

References

Dinarello, C.A. and P. Bufler (2013) Sem. Immunol. 25:466.
Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
Ding, V.A. et al. (2016) Med. Oncol. 33:68.
Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
Kumar S. et al. (2000) J. Biol Chem 275:10308.
Busfield S.J. et al. (2000) Genomics 66:213.
Pan, G. et al. (2000) Cytokine 13:1.
Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
Kumar S. et al. (2002) Cytokine 18:61.
Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
Sharma, S. et al. (2008) J. Immunol. 180:5477.

Long Name

Interleukin 37

Entrez Gene IDs

27178 (Human)

Alternate Names

FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein

Related Research Areas

IL-1 Family

Citation for Recombinant Human IL-37/IL-1F7 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1