Recombinant Human LIF, Animal-Free Protein

Catalog #: Qk036 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
Qk036-0050
Qk036-0500

Recombinant Human LIF, Animal-Free Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LIF, Animal-Free Protein Summary

Product Specifications

Accession #

P15018.1

Product Datasheets

QK036

Product Datasheet

COA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Qk036

Stability & Storage:

Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Scientific Data

Bioactivity

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Human LIF activity is determined using the LIF-responsive firefly luciferase reporter assay (*). HEK293T cells are treated in triplicate with a serial dilution of LIF overnight. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0.29 ng/ml (17.3 pM).*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

SDS-PAGE

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Human LIF protein (Qk036) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R). Purified recombinant human LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Reconstitution Calculator

Background: LIF

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.

Long Name

Leukemia Inhibitory Factor

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Alternate Names

CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI