Recombinant Human LILRB1/CD85j/ILT2 Fc Biotin Protein, CF

Catalog #: BT2017 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
BT2017-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LILRB1/CD85j/ILT2 Fc Biotin Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human LILRB1/CD85j/ILT2 Antibody (Catalog # MAB20172) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # BT2017) binds with an ED₅₀ of 1.50-18.0 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human LILRB1/CD85j/ILT2 protein

Human LILRB1/CD85j/ILT2 (Gly24-His458) Accession # Q8NHL6.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8NHL6.1

N-terminal Sequence
Analysis

Gly24

Structure / Form

Disulfide-linked homodimer

Biotinylated via amines

Predicted Molecular Mass

74 kDa

SDS-PAGE

93-107 kDa, under reducing conditions.

Product Datasheets

BT2017

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT2017

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human LILRB1/CD85j/ILT2 Antibody (MAB20172) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein (Catalog # BT2017) binds with an ED₅₀ of 1.50-18.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Biotinylated Protein (Catalog # BT2017) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93-107 kDa and 190-210 kDa, respectively.

Reconstitution Calculator

Background: LILRB1/CD85j/ILT2

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HC-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).

References

Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
Harly, C. et al. (2011) Blood 117:2864.
Cosman, D. et al. (1997) Immunity 7:273.
Young, N.T. et al. (2001) J. Immunol. 166:3933.
Young, N.T. et al. (2008) Blood 111:3090.
Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
Allen, R.L. et al. (2001) J. Immunol. 167:5543.
Ince, M.N. et al. (2004) Immunology 112:531.
Saverino, D. et al. (2000) J. Immunol. 165:3742.
Saverino, D. et al. (2002) J. Immunol. 168:207.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1

Entrez Gene IDs

10859 (Human)

Alternate Names

CD85 antigen-like family member J; CD85j antigen; CD85j; Ig-like transcript 2; ILT2; ILT-2; ILT2FLJ37515; Immunoglobulin-like transcript 2; leukocyte Ig-like receptor-1; Leukocyte immunoglobulin-like receptor 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB1; LIR1; LIR1LIR-1MIR-7CD85MIR7CD85 antigen; member 1; MIR7; Monocyte/macrophage immunoglobulin-like receptor 7