Recombinant Human MEGF9 His-tag Protein, CF

Catalog #: 10514-ME Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10514-ME-050

Recombinant Human MEGF9 His-tag Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MEGF9 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human MEGF9 His-tag, immobilized at 0.15 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Source

Mouse myeloma cell line, NS0-derived human MEGF9 protein
Ala36-Asn514, with a C-terminal 6-His tag

Accession #

Q9H1U4.3

N-terminal Sequence
Analysis

Ala36

Predicted Molecular Mass

51 kDa

SDS-PAGE

136-150 kDa, under reducing conditions

Product Datasheets

10514-ME

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10514-ME

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human MEGF9 His-tag (Catalog # 10514-ME) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 136-150 kDa.

Reconstitution Calculator

Background: MEGF9

MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a novel transmembrane glycoprotein with multiple EGF-like repeats. It is predominantly expressed in the developing and adult central nervous system and peripheral nervous system. Immunohistochemical studies showed that MEGF9 is expressed in Purkinje cells of the cerebellum and in glial cells of the peripheral nervous system. In non-neuronal tissues, MEGF9 is expressed in epidermal layer of skin, papillae of the tongue and epithelium of the gastrointestinal tract. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular domain (ECD). The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200). N-terminal region of MEGF9 includes several potential O-glycosylation sites followed by five EGF-like domains which are highly homologous to the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short intracellular domain with potential phosphorylation sites is present (1, 2). Within the ECD, human MEGF9 shares 75% aa sequence identity with mouse and rat MEGF9.

References

Brandt-Bohne, U. et al. (2007) Biochem. J. 401:447.
Heller, B. et al. (2014) J. Cell. Biol. 204:1219.

Long Name

Multiple EGF-like-domains 9

Entrez Gene IDs

1955 (Human); 230316 (Mouse); 313270 (Rat)

Alternate Names

EGFL5; EGFL5KIAA0818; EGF-like protein 5; EGF-like-domain, multiple 5; Epidermal growth factor-like protein 5; MEGF9; Multiple EGF-like domains protein 9; multiple EGF-like-domains 9; multiple epidermal growth factor-like domains protein 9