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Recombinant Human/Mouse/Rat NEDD8 Protein, CF

Catalog #: UL-812
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
UL-812-500
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Mouse/Rat NEDD8 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human NEDD8 can be conjugated to substrate proteins via the subsequent actions of a NEDD8-activating (E1) enzyme, a NEDD8-conjugating (E2) enzyme, and a NEDD8 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 concentration of 10-50 μM.
Source
E. coli-derived NEDD8 protein
Met1 - Gly76
Accession #
Q15843.1
Predicted Molecular Mass
8.6 kDa

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UL-812

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-812

Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD8

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 is highly conserved and shows 100% aa identity to NEDD8 from several species including mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

References
  1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393. 
  2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
  3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
  4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
  5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
  7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
  8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
  9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
  10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
  11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
  12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

Citations for Recombinant Human/Mouse/Rat NEDD8 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Mono-ubiquitination empowers ubiquitin chain elongation
    Authors: Wu, K;DeVita, RJ;Pan, ZQ;
    The Journal of biological chemistry
    Species: Human hepegivirus
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair
    Authors: Kokic, G;Yakoub, G;van den Heuvel, D;Wondergem, AP;van der Meer, PJ;van der Weegen, Y;Chernev, A;Fianu, I;Fokkens, TJ;Lorenz, S;Urlaub, H;Cramer, P;Luijsterburg, MS;
    Nature structural & molecular biology
    Species: olive baboon
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses
    Authors: S Banchenko, F Krupp, C Gotthold, J Bürger, A Graziadei, FJ O'Reilly, L Sinn, O Ruda, J Rappsilber, CMT Spahn, T Mielke, IA Taylor, D Schwefel
    PloS Pathogens, 2021-08-02;17(8):e1009775.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human, N/A
    Sample Types: Peptide, Protein
    Applications: Bioassay
  5. Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression
    Authors: X Liu, JM Reitsma, JL Mamrosh, Y Zhang, R Straube, RJ Deshaies
    Mol. Cell, 2018-03-01;69(5):773-786.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  6. A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation
    Authors: H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S Wang
    Nat Commun, 2017-10-27;8(1):1150.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity.
    Authors: Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh B
    J Biol Chem, 2014-11-19;290(1):296-309.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  8. SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1).
    Authors: Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, Buss E, Shaha M, Rechler W, Ramanathan S, Singh B
    2014-10-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  9. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.
    Authors: Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross J
    PLoS Pathog, 2012-12-27;8(12):e1003085.
    Applications: Bioassay

FAQs

  1. Does Recombinant Human NEDD8 (Catalog # UL-812) have a tag?

    • Recombinant Human NEDD8 (Catalog # UL-812) does not have a tag.  Please refer to the Source section on the product-specific page for sequence information.

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