Recombinant Human Neurturin (Histidine-tagged) Protein

Catalog #: 387-NE Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
387-NE-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
387-NE-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Neurturin (Histidine-tagged) Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED₅₀ for this effect is 2‑6 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized rhGFR alpha 2/Fc Chimera at 1 µg/mL (100 µL/well) can bind hNeurturin with a linear range of 0.5-40 ng/mL.

Source

E. coli-derived human Neurturin protein

ATVID	10-His tag	SSNG	Human Neurturin (Ala96 - Val197) Accession # Q99748
N-terminus			C-terminus

Accession #

Q99748

N-terminal Sequence
Analysis

Ala

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

14 kDa (monomer)

Product Datasheets

387-NE (with carrier)

Product Datasheet

COA

SDS

387-NE/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

387-NE

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

387-NE/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Neurturin

Neurturin (NTN) is a member of the GDNF family of ligands which also includes glial cell-derived neurotrophic factor (GDNF), persephin, and artemin. GDNF family proteins are distant members of the TGF-beta superfamily and contain a conserved seven cysteine motif found in the entire family. Human NTN encodes a 197 amino acid (aa) preproprotein with a 19 aa residue putative signal peptide and a 76 aa pro region. Proteolytic cleavage of the pro-protein occurs at an RXXR consensus sequence. The native protein is a disulfide-linked homodimer with a calculated monomeric mass of about 12.5 kDa. The amino acid sequence of human NTN shares 91% identity to mouse NTN. NTN also shares about 42% similarity with GDNF. The bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1 - GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase). NTN prefers to bind GFR alpha 2 but can also bind GFR alpha 1. NTN can promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. The wide expression of NTN in both neuronal and non-neuronal tissues suggests that NTN may regulate the development and maintenance of the central and peripheral nervous systems and other systems.

References