Recombinant Human NKp30 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI1849 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI1849-050

Recombinant Human NKp30 Fc Chimera Avi-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NKp30 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 Fc Chimera (Catalog # 7144-B7) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human NKp30/NCR3 Fc Chimera Avi-tag binds with an ED₅₀ of 8.00-96.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human NKp30/NCR3 protein

Human NKp30 (Leu19-Thr138) Accession # Q14931.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

Q14931.1

N-terminal Sequence
Analysis

Leu19

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

53-63 kDa, under reducing conditions.

Product Datasheets

AVI1849

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1849

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human B7-H6 Fc Chimera (7144-B7) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human NKp30 Fc Chimera Avi-tag binds with an ED50 of 8.00-96.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human NKp30 Fc Chimera Avi-tag Protein (Catalog # AVI1849) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-63 kDa and 110-120 kDa, respectively.

Reconstitution Calculator

Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (1). These receptors play a major role in triggering NK-mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM‑bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56^dim, CD16⁺ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56^bright, CD16^- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described. Our Avi-tag Biotinylated human NKp30 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.

Entrez Gene IDs

259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)

Alternate Names

Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS