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Recombinant Human NKp44 Fc Chimera Protein, CF

Catalog #: 2249-NK
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2249-NK-050
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Citations (15)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NKp44 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind HeLa human cervical epithelial carcinoma cells in a flow cytometry assay. When 400 ng of rhNKp44 Fc chimera is added to 2 x 105 Hela cells, >25% cells bind to the protein.
Source
Mouse myeloma cell line, NS0-derived human NKp44/NCR2 protein
Human NKp44
(Gln22 - Pro190)
Accession # CAB31968.1		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
CAB31968.1
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa (monomer)
SDS-PAGE
61-67 kDa, reducing conditions

Product Datasheets

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2249-NK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2249-NK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKp44/NCR2

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
  5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
Long Name
NK Cell Activating Receptor
Entrez Gene IDs
9436 (Human); 102129442 (Cynomolgus Monkey)
Alternate Names
CD336 antigen; CD336; LY95; LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NCR2; NK cell activating receptor (NKp44); NK cell-activating receptor; NKp44; NK-p44dJ149M18.1

Citations for Recombinant Human NKp44 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma
    Authors: Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai
    Translational Oncology, 2022-08-20;25(0):101521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Hypoxia-Driven HIF-1&alpha Activation Reprograms Pre-Activated NK Cells towards Highly Potent Effector Phenotypes via ERK/STAT3 Pathways
    Authors: SA Lim, Y Moon, MH Shin, TJ Kim, S Chae, C Yee, D Hwang, H Park, KM Lee
    Cancers, 2021-04-15;13(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function
    Authors: A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard
    BMC Immunol., 2019-01-29;20(1):8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation
    Proc Natl Acad Sci USA, 2016-05-09;113(21):E2973-82.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  5. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
    Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
    PLoS ONE, 2015-05-07;10(5):e0125544.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
    Authors: Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming
    Sci Rep, 2014-08-20;4(0):6138.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014-04-30;192(11):5332-42.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  8. Human cytomegalovirus infection elicits new decidual natural killer cell effector functions.
    Authors: Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N
    PLoS Pathog, 2013-04-04;9(4):e1003257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Engagement of Siglec-7 receptor induces a pro-inflammatory response selectively in monocytes.
    Authors: Varchetta S, Brunetta E, Roberto A, Mikulak J, Hudspeth K, Mondelli M, Mavilio D
    PLoS ONE, 2012-09-28;7(9):e45821.
    Species: Bacteria - E. Coli, C. albicans
    Sample Types: Whole Cells
    Applications: Binding Assay
  10. Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells.
    Authors: Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J
    J. Clin. Invest., 2012-06-11;122(7):2369-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012-06-06;86(16):8693-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Direct binding of human NK cell natural cytotoxicity receptor NKp44 to the surfaces of mycobacteria and other bacteria.
    Authors: Esin S, Batoni G, Counoupas C, Stringaro A, Brancatisano FL, Colone M, Maisetta G, Florio W, Arancia G, Campa M
    Infect. Immun., 2008-01-22;76(4):1719-27.
    Species: Bacteria
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007-10-18;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells.
    Authors: Banerjee P, Feuer G, Barker E
    J. Virol., 2007-07-03;81(18):9707-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
    Authors: Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, Barker E
    Blood, 2007-05-18;110(4):1207-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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