Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF

Catalog #: 10371-NK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10371-NK-050

Recombinant Human NKp65/KLRF2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NKp65/KLRF2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) is immobilized 0.1 μg the concentration of Recombinant Human CLEC-2A (Catalog # 8435-CL) the optimal binding response is approximately 0.5-3 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human NKp65/KLRF2 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human NKp65/KLRF2 (Asp52-Val156) Accession # D3W0D1.1
N-terminus			C-terminus

Accession #

D3W0D1.1

N-terminal Sequence
Analysis

Met Asp - Pro100

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa

SDS-PAGE

50-65 kDa, under reducing conditions

Product Datasheets

10371-NK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10371-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) is immobilized at 0.1 µg/mL, 100 µL/well, the concentration for Recombinant Human CLEC-2A (Catalog # 8435-CL) that produces 50% of the optimal binding response is approximately 0.5-3 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human NKp65/KLRF2 Fc Chimera (Catalog # 10371-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa and 100-130 kDa, respectively.

Reconstitution Calculator

Background: NKp65/KLRF2

Killer cell lectin-like receptor subfamily F member 2, also known as KLRF2 and NKp65, is a C-type lectin-like receptor and a member of the NKRP1 (NK receptor protein) subfamily (1-3). KLRF2, along with the related KLRF1, is an activating receptor for the C-type-lectin-like-2 (CLEC2) family of ligands (1-3). KLRF2 is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic domain, a single transmembrane region and an extracellular domain (ECD) consisting of 26-residue stalk and 130-residue C-type lectin-like domain (CTLD) (1, 3). Unlike other members of the NKRP1 family such as KLRF1, KLRF2 is a non-disulfide-linked homodimer (1, 2). While sharing subfamily-specific traits classifying them as members of the NKRP1 subfamily, there does not appear to be a direct homolog of KLRF2 in mouse (2). KLRF2 is minimally expressed on peripheral blood NK cells and its ligand, Keratinocyte-associated C-type lectin (KACL), is restricted to human keratinocytes (1-3). This expression of KACL indicates potential implications in skin-affecting diseases such as psoriasis and wound healing (1). The signaling pathway of KLRF2 is distinct from most NK receptors because it does not utilize immunoreceptor tyrosine-based activating motif (ITAM)-containing signaling adaptors, but rather uses an activating signaling motif with only one tyrosine module referred to as hemITAM (1, 3).

References