Recombinant Human Oncostatin M (OSM), Animal-Free Protein

Catalog #: Qk049 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
Qk049-0050
Qk049-0500

Recombinant Human Oncostatin M (OSM), Animal-Free Protein Bioactivity

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All Oncostatin M/OSM Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Oncostatin M (OSM), Animal-Free Protein Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

No significant difference between EC₅₀ of reference and test lots

Source

E. coli-derived human Oncostatin M/OSM protein

Accession #

P13725.2

Predicted Molecular Mass

22 kDa

SDS-PAGE

Monomeric Oncostatin M (OSM) protein only

Product Datasheets

QK049

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Qk049

Formulation	Lyophilized from acetonitrile/TFA
Reconstitution	Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired.
Shipping	The product is shipped lyophilized at ambient temperture, on ice blocks or dry ice. Shipping at ambient temperture does not affect the bioactivity or stability of the protein. Upon reciept, store immediately at the conditions stated below.
Stability & Storage:	Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles.

Scientific Data

Bioactivity

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OSM activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of OSM for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC₅₀ = 0.75 ng/ml (34.2 pM).*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

SDS-PAGE

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OSM migrates as a single band at 20 kDa in non-reducing (NR) conditions and 22 kDa upon reduction (R). The slight band under the main band in the reduced sample is likely an artefact of the reduction protocol. No contaminating protein bands are visible.Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.

Reconstitution Calculator

Background: Oncostatin M/OSM

Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development (1). The human OSM cDNA encodes a 252 amino acid (aa) prepro protein with a signal peptide and a hydrophilic Cterminal domain that are proteolytically processed to generate the 195 aa mature form (2). Although both mature and pro-OSM are equally active in radioreceptor assays, the mature OSM is 5 to 60 fold more active in growth inhibition assays (3). Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM (3). Cytokines of the IL-6 family share a common receptor subunit, gp130, which allows intracellular signal transduction. In contrast to other cytokines, OSM binds gp130 with very low affinity and has little to no biological activity unless a second receptor chain is recruited (4). The OSM-gp130 complex recruits either LIF R alpha, or OSM R beta to form the OSM receptor type I or OSM receptor type II respectively (5). Among multiples roles, OSM has been reported to modulate tumor cell and epithelial cell growth (6, 7). An axis involving OSM, CD40 Ligand and OSM receptor described as an antiviral and immunostimulatory system is disrupted in patients liver showing hepatitis C infection (8). Human OSM shares 45% aa sequence homology with mouse and rat OSM.

References

Richards, C.D. (2013) ISRN Inflamm. 2013:512103.
Malik, N. et al. (1989) Mol. Cel. Biol. 9:2847.
Linsley, P.S. et al. (1990) Mol. Cel. Biol. 10:1882.
Liu, J. et al. (1994) Cytokine 6:272.
Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
Grant, S.L. et al. (2001) Growth Factors 19:153.
Chollangi, S. et al. (2012) J. Biol. Chem. 287:32848.
Larrea, E. et al. (2014) J. Hepatol. 60:482.

Entrez Gene IDs

5008 (Human); 18413 (Mouse)

Alternate Names

MGC20461; oncostatin M; oncostatin-M; OSM

Product Specific Notices

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.