Recombinant Human PEAR1 Protein, CF

Catalog #: 4527-PR
2 Citations Datasheet / COA / SDS

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4527-PR-050

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Citations (2)

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Recombinant Human PEAR1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of thrombin-activated human platelets. When 5 x 10 6 cells per well are added to rhPEAR1 coated plates (1 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 1 hour at 37 degreesC.

Source

Chinese Hamster Ovary cell line, CHO-derived human PEAR1 protein Leu21-Ser754 with a C-terminal 6-His tag

Accession #

NP_001073940

N-terminal Sequence
Analysis

Leu21

Predicted Molecular Mass

78.3 kDa

SDS-PAGE

120-130 kDa, reducing conditions

Product Datasheets

4527-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4527-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Reconstitution Calculator

Background: PEAR1

Platelet endothelial aggregation receptor 1 (PEAR1) is a 150 kDa type I transmembrane protein and member of the MEGF family of proteins (1). Human PEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20 aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 261 aa cytoplasmic region (SwissProt # Q5VY43). The ECD consists of 15 EGF-like repeats that vary in length from 39 to 42 aa and contain a consensus sequence of CX 1-2 GX 2 GX 2-4 CX 3 CX 1-3 CX 1-2 GX 1-2 CX 4 GX 1 CX 1 CX 2 GX 2 GX 2 C (1). The consensus repeat contains six conserved glycine residues and eight conserved cysteine residues, suggesting four disulfide-bonded cysteine pairs in each EGF repeat (1). Within the ECD, there are also five potential sites for N-linked glycosylation. The cytoplasmic region contains five potential Src homology 3-binding, proline-rich domains (1). Mature human PEAR1 is 84% aa identical to mature mouse PEAR1. PEAR1 is most highly expressed in platelets and endothelial cells (1). Functionally, PEAR1 is a receptor for a yet undetermined ligand that signals upon the formation of platelet-platelet contacts induced both by platelet aggregations and by platelet centrifugation (1). The signaling enhances and stabilizes platelet thrombi (2). Upon aggregation, the surface-expressed protein is tyrosine-phosphorylated (1). This phosphorylation event is inhibited by the alpha IIb beta 3 antagonist eptifibatide, thus demonstrating that PEAR1 tyrosine phosphorylation is dependent on surface contacts between activated platelets (1). PEAR1 can be phosphorylated in an alpha IIb beta 3 integrin-dependent manner on tyrosine (Tyr925) and serine residues (Ser593 and Ser1029) and, potentially, at Tyr804, Tyr943, and Tyr979 (1). Inherited PEAR1 variations that alter expression or function of the platelet signaling molecule could modify agonist-induced aggregation in native platelets (2). In addition, a genetic variant in PEAR1 could be an important determinant of residual platelet function during aspirin treatment, because the COX1/thromboxane A2 pathway will be strongly inhibited by aspirin, and maximal aggregation will then be dependent on other secondary signaling pathways (2).

References

Nanda, N. et al. (2005) J. Biol. Chem. 280:24680.
Herrera-Galeano, J.E. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1484.

Long Name

Platelet endothelial aggregation receptor 1

Entrez Gene IDs

375033 (Human); 73182 (Mouse)

Alternate Names

Jedi; PEAR1; platelet endothelial aggregation receptor 1

Related Research Areas

Citations for Recombinant Human PEAR1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A novel mechanism of sustained platelet ÃŽÂ±IIbÃŽÂ²3 activation via PEAR1.
Authors: Kauskot A, Di Michele M, Loyen S, Freson K, Verhamme P, Hoylaerts MF
Blood, 2012-02-27;119(17):4056-65.
Species: Human
Sample Types: Platelet Rich Plasma (PRP)
Applications: Bioassay
Identification of a specific intronic PEAR1 gene variant associated with greater platelet aggregability and protein expression.
Authors: Faraday N, Yanek LR, Yang XP, Mathias R, Herrera-Galeano JE, Suktitipat B, Qayyum R, Johnson AD, Chen MH, Tofler GH, Ruczinski I, Friedman AD, Gylfason A, Thorsteinsdottir U, Bray PF, O'Donnell CJ, Becker DM, Becker LC
Blood, 2011-07-26;118(12):3367-75.
Applications: ELISA (Standard)