Recombinant Human Periostin/OSF-2 (C60A) His-tag Protein, CF New

Catalog #: 11486-F2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11486-F2-050
11486-F2-500

Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Periostin/OSF-2 (C60A) His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.500-6.00 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human Periostin/OSF-2 protein
Asn22-Gln808 (C60A) with a C-terminal 6-His tag

Accession #

Q15063.1

N-terminal Sequence
Analysis

Asn22

Predicted Molecular Mass

89 kDa

SDS-PAGE

68-90 kDa, under reducing conditions.

Product Datasheets

11486-F2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11486-F2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein (Catalog # 11486-F2) supports the adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.500‑6.00 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Periostin/OSF‑2 (C60A) His-tag Protein (Catalog # 11486-F2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-90 kDa, under reducing conditions.

Reconstitution Calculator

Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature human Periostin shares 91% amino acid sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). Periostin is also up-regulated in many carcinomas (2, 8) and induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Further, Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

References

Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Li, G. et al. (2006) Atherosclerosis 188:292.
Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
Erkan, M. et al. (2007) Gastroenterology 132:1447.
Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
Kudo, Y. et al. (2012) PLoS One 7:e44488.
Snider, P. et al. (2008) Circ. Res. 102:752.
Kuhn, B. et al. (2007) Nat. Med. 13:962.
Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
Sehra, S. et al. (2011) J. Immunol. 186:4959.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52