Recombinant Human S100A1 Protein, CF

Catalog #: 9705-S1 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9705-S1-100

Recombinant Human S100A1 Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human S100A1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human S100A1 is immobilize at 1 μg/mL, 100 μL/well, it binds recombinant human HSP70/HSPA1A. The concentration of recombinant human HSP70/HSPA1A that produces 50% of the optimal binding response is 0.8-4.8 μg/mL.

Source

E. coli-derived human S100A1 protein
Met1-Ser94

Accession #

P23297

N-terminal Sequence
Analysis

Met1 & Gly2

Predicted Molecular Mass

11 kDa

SDS-PAGE

7 kDa, reducing conditions

Product Datasheets

9705-S1

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9705-S1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human S100A1 (Catalog # 9705-S1) is coated at 1 μg/mL, recombinant human HSP70/HSPA1A binds with an ED₅₀ of 0.8-4.8 μg/mL.

Reconstitution Calculator

Background: S100A1

S100A1, also known as S100 Alpha, is a 10 kDa member of the S100 (soluble in 100% ammonium sulfate at neutral pH) family of proteins (1-5). The S100 family is further classified as a member of the EF-hand superfamily of Ca2⁺ binding protein. The hallmark of this superfamily is the EF-hand motif that consists of a Ca2⁺‑binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein (6). S100A1 can also bind zinc in vitro and its binding sites are different from the calcium binding sites. Typically, S100A1 exists as homodimers but it can also form non-covalent heterodimers with other members of the S100 family (7, 8). Human S100A1 is 94 amino acids (aa) in length. It shares 93%, 94%, 98% and 100% aa sequence identity with mouse, rat, bovine and Rhesus macaque respectively (9). Within the S100 family, S100A1 shows the highest aa identity (56%) with S100B (10). S100 A1 is highly expressed in the myocardium, and it is an important regulator of the cardiac contractility (11, 12). S100A1 acts as a Ca2⁺ signal transducer (13). In response to an increase in intracellular Ca2⁺levels, S100 A1 binds calcium which triggers a conformational change (14). This conformational change allows interaction of S100A1 with specific target proteins, such as TPR-containing proteins, and the modulation of their activity (13). S100A1 also acts as a cardioprotective factor to inhibit apoptosis in neonatal ventricular cardiomyocytes via activation of the ERK1/2 (12).

References

Engelkamp, D. et al. (1992) Biochemistry 31:10258.
Tomida, Y. et al. (1992) Biochem. Biophys. Res. Commun. 189:1310.
Garrett, S.C. et al. (2006) J. Biol. Chem. 281:677.
Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
Donato, R. (2001) Int. J. Biochem. Cell. Biol. 33:637.
Kretsinger, R.H. and C.E. Nockolds (1973) J. Biol. Chem. 248:3313.
Wang, G. et al. (2004) Biochem. J. 382:375.
Wang, G. et al. (2000) J. Biol. Chem. 275:11141.
Ridinger, K et al. (1998) Biochem. Biophys. Acta 1448:254.
Donato R. et al. (1999) Braz J Med Biol Res. 32(10):1177.
Duarte-Costa, S. et al. (2014) Physiol. Res. 63:669
Most, P. et al. (2003) J. Biol. Chem. 278:48404.
Yamaguchi F. et al. (2012) J. Biol. Chem. 287:13787.
Nowakowski M. et al. (2013) Biochemistry 52:1149.

Long Name

S100 Calcium Binding Protein A1

Entrez Gene IDs

6271 (Human); 20193 (Mouse); 295214 (Rat)

Alternate Names

protein S100-A1; S100 alpha; S100 calcium binding protein A1; S100 calcium-binding protein A1S100; S-100 protein alpha chain; S-100 protein subunit alpha; S100 protein, alpha polypeptide; S100A; S100A1; S100-alpha