Recombinant Human SHBG Protein, CF

Catalog #: 9614-SG Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9614-SG-050

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human SHBG Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human SHBG protein
Leu30-His402, with a C-terminal 6-His tag

Accession #

P04278-1

N-terminal Sequence
Analysis

Leu30

Predicted Molecular Mass

41 kDa

SDS-PAGE

41-52 kDa, reducing conditions

Product Datasheets

9614-SG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9614-SG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SHBG

Human SHBG (Sex hormone-binding globulin), also known as ABP (Testis-specific Androgen-binding protein) or TeBG (Testosterone-estradiol-binding globulin), is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family (1). Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G‑ like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G‑ like domain (2). SHBG is synthesized by the liver and circulates in blood. It binds to certain androgens and estrogens and serves as a transporter for the bound steroids (3); it is also involved in receptor mediated processes (4). Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG. Low SHGB levels are associated with increased risk for type 2 diabetes for both man and women (5).

References

Awakumov G.V. et al. (2010) Mol. Cell Endocrinol. 5:316.
Grishkovskaya I. et al. (2000) EMBO J. 19:504.
Anderson D.C. (1974) Clin. Endocrinol. (Oxf.) 3:69.
Rosner W. et al. (2010) Mol. Cell Endocrinol.5:795.
Muka T. et al. (2017) Diabetes. 66:577.

Long Name

Sex Hormone-binding Globulin

Entrez Gene IDs

6462 (Human)

Alternate Names

ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin