Recombinant Human Tau 412 (1N4R) Protein, CF

Catalog #: SP-501 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
SP-501-100

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Tau 412 (1N4R) Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Concentrations for in vitro assays will depend on experimental conditions and detection methods.

Source

E. coli-derived human Tau protein
Met1 - Leu412

Accession #

NP_001116539.1

Predicted Molecular Mass

43 kDa

Product Datasheets

SP-501

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-501

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Tau

Tau is a microtubule-associated protein expressed primarily in neurons. Carboxy-terminal domains of Tau associate with and stabilize microtubule structure, while other domains bind to the plasma membrane. Abnormal Tau phosphorylation may result in the self-assembly of tangles of paired helical and/or straight filaments, which are involved in the pathogenesis of Alzheimer's disease and other neurodegenerative diseases. Properly folded Tau is highly soluble, but when the protein becomes misfolded it forms insoluble aggregates that can damage cytoplasmic functions, interfere with axonal transport and ultimately lead to cell death. There are multiple forms of Tau. The product is a 412 amino acid isoform known as "1N4R," "Isoform Tau-E" or "Tau 412" and is also referenced in UniProt as P10636-7.

References

Billingsley M.L. & Kincaid R.L. (1997) Biochem. J. 323:577.
Bloom G.S. (2014) JAMA Neurol. 71:505.
Cripps D. et al. (2006) J. Biol. Chem. 281:10825.
Harada A. et al. (1994) Nature 369:488.
Lei P. et al. (2010) Int. J. Biochem. Cell Biol. 42:1775.

Long Name

Microtubule-Associated Protein Tau

Entrez Gene IDs

4137 (Human); 17762 (Mouse); 29477 (Rat)

Alternate Names

DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MGC138549; microtubule-associated protein tau; MSTD; MSTDMAPTL; MTBT1; MTBT1Neurofibrillary tangle protein; MTBT2; Neurofibrillary tangle protein; PHF-tau; PPND; Tau; TAUPaired helical filament-tau