Recombinant Human TIM-3 His-tag Avi-tag Protein, CF

Catalog #: AVI10241 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10241-050

Recombinant Human TIM-3 His-tag Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TIM-3 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin‑9 (Catalog # 2045-GA) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human TIM‑3 His-tag Avi-tag (Catalog # AVI10241) that produces 50% of the optimal binding response is 0.1-0.8 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human TIM-3 protein

Human TIM-3 (Ser22-Arg200) Accession # AAH63431.1	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

AAH63431.1

N-terminal Sequence
Analysis

Ser22

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

23 kDa

SDS-PAGE

37-50 kDa, under reducing conditions

Product Datasheets

AVI10241

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10241

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Galectin-9 (Catalog # 2045-GA) is immobilized at 1 µg/mL, 100 µL/well, Biotinylated Recombinant Human TIM-3 His-tag Avi-tag (AVI10241) binds with an ED₅₀ of 0.1-0.8 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TIM-3 His-tag Avi-tag (Catalog # AVI10241) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-50 kDa.

Reconstitution Calculator

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8⁺, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8⁺ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References

Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
Monney, L. et al. (2002) Nature 415:536.
Nakayama, M. et al. (2009) Blood 113:3821.
Anderson, A.C. et al. (2007) Science 318:1141.
Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
Chiba, S. et al. (2012) Nat. Immunol. 13:832.
Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
Ndhlovu, L.C. et al. (2012) Blood 119:3734.
Gleason, M.K. et al. (2012) Blood 119:3064.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
Dardalhon, V. et al. (2010) J. Immunol. 185:1383.

Long Name

T Cell Immunoglobulin Mucin-3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Alternate Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3