Recombinant Human TrkA Fc Chimera Protein, CF

Catalog #: 175-TK
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
175-TK-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (8)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TrkA Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.8-4 µg/mL in the presence of 10 ng/mL of rhNGF.

Source

Mouse myeloma cell line, NS0-derived human TrkA protein

Human TrkA (Ala33-Glu407) Accession #AAA36770	IEGRID	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAA36770

N-terminal Sequence
Analysis

Ala33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

67.4 kDa (monomer)

SDS-PAGE

115-125 kDa, reducing conditions

Product Datasheets

175-TK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

175-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

References

Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat)

Alternate Names

DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

Related Research Areas

Citations for Recombinant Human TrkA Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics
Authors: AJ Poole, L Frigotto, ME Smith, C Baar, G Ivanova-Be, A Jaulent, C Stace, CG Ullman, AV Hine
Sci Rep, 2019-01-30;9(1):930.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA (Capture)
Nerve Growth Factor Is Responsible for Exercise-Induced Recovery of Septohippocampal Cholinergic Structure and Function
Authors: JM Hall, F Gomez-Pini, LM Savage
Front Neurosci, 2018-11-01;12(0):773.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus
Authors: JI Hurtado-Za, B Ramachandr, S Ahmed, R Halder, C Bolleyer, A Awasthi, MA Stahlberg, RJ Wagener, K Anderson, RM Drenan, HA Lester, JM Miwa, JF Staiger, A Fischer, C Dean
Nat Commun, 2017-07-19;8(0):15878.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations
Authors: JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo
Proc. Natl. Acad. Sci. U.S.A, 2017-03-28;0(0):.
Applications: Bioassay
Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
Authors: Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
J Immunol, 2014-02-28;192(7):3345-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Brain-derived neurotrophic factor ameliorates brain stem cardiovascular dysregulation during experimental temporal lobe status epilepticus.
Authors: Tsai CY, Chan JY, Hsu KS
PLoS ONE, 2012-03-19;7(3):e33527.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
Authors: Vaegter CB, Jansen P, Fjorback AW
Nat. Neurosci., 2010-11-21;14(1):54-61.
Applications: Surface Plasmon Resonance
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
Authors: Lu B, PereiraPerrin M
Infect. Immun., 2008-06-09;76(8):3530-8.
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP