Recombinant Human TROP-2 His-tag Protein, CF

Catalog #: 11158-T2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11158-T2-100

Recombinant Human TROP-2 His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TROP-2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IGF-II R/IGF2R (Catalog # 6418-GR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human TROP-2 His-tag (Catalog # 11158-T2) binds with an ED₅₀ of 20.0-200 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human TROP-2 protein
His27-Thr274, with a C-terminal 6-His tag

Accession #

P09758.3

N-terminal Sequence
Analysis

His27

Predicted Molecular Mass

29 kDa

SDS-PAGE

32-57 kDa, under reducing conditions.

Product Datasheets

11158-T2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11158-T2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IGF-II R/IGF2R Protein (6418-GR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human TROP-2 His-tag Protein (Catalog # 11158-T2) binds with an ED₅₀ of 20.0-200 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TROP-2 His-tag Protein (Catalog # 11158-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-57 kDa.

Reconstitution Calculator

Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is transducing an intracellular calcium signal and may play a role in tumor growth. TROP-2 is associated with EGF mutation and resistance to drug treatment in many tumors. It has been discovered to bind to IGF IIR and promote IGF2-IGF1R-Akt (6). TROP-2 has emerged as a novel cancer target and a target for antibody-drug conjugates (7). TROP-2 interacts with ADAM-10 and binds E-Cadherin stimulating ADAM-10 mediated cleavage (8).

References

Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
Ripani, E. et al. (1998) Int. J. Cancer 76:671.
El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Sun, X. et al. (2021) J. Cancer 12:5310.
Goldenberg, D. M. et al. (2018) Oncotarget. 9:28989.
Guerra, E. et al. (2021) Neoplasia. 23:898.

Long Name

Tumor-associated Calcium Signal Transducer 2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Alternate Names

Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2