Recombinant Human TSPAN14-LEL Fc Chimera Protein, CF New

Catalog #: 11552-TS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11552-TS-100

Recombinant Human TSPAN14-LEL Fc Chimera Protein SDS-PAGE.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TSPAN14-LEL Fc Chimera Protein, CF Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Human embryonic kidney cell, HEK293-derived human TSPAN14 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human TSPAN14-LEL (LEU114-Asn232) Accession # Q8NG11.1
N-terminus			C-terminus

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

40 kDa

SDS-PAGE

43-54 kDa, under reducing conditions

Product Datasheets

11552-TS

Product Datasheet

COA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11552-TS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human TSPAN14-LEL Fc Chimera Protein (Catalog # 11552-TS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-54 kDa and 90-110 kDa, respectively.

Reconstitution Calculator

Background: TSPAN14

Tetraspanin 14, or TSPAN14, belongs to a superfamily of proteins that is characterized by four transmembrane domains, three intracellular domains and two extracellular loops: a small extracellular loop (SEL) and a large extracellular loop (LEL). The extracellular loops form molecular webs that bring together cell surface proteins, facilitating the formation of stable and functional signalling complexes. Tetraspanins form microdomains on the plasma membrane that mediate diverse biological processes including adhesion, cell fusion, immune response, and tumor development (1-4). Human TSPAN14 consists of 270 amino acids, with the LEL region spanning residues 114-232. Within the LEL, human TSPAN14 shares 98% aa sequence identity with mouse and rat TSPAN14-LEL.

References