Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

Catalog #: 938-VC
4 Citations Datasheet / COA / SDS

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938-VC-050

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 40-160 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived human VE-Cadherin protein

Human VE-Cadherin (Asp48-Gln593) Accession # P33151	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

P33151

N-terminal Sequence
Analysis

Asp48

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

89 kDa (monomer)

SDS-PAGE

116 kDa, reducing conditions

Product Datasheets

938-VC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

938-VC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: VE-Cadherin

VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins (1). Mature human VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca⁺⁺-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).

References

Giannotta, M. et al. (2013) Dev. Cell 26:441.
Breviario, F. et al. (1995) Arterioscler. Throm. Vasc. Biol. 15:1229.
Geyer, H. et al. (1999) Glycobiology 9:915.
Herren, B. et al. (1998) Mol. Biol. Cell 9:1589.
Li, H. et al. (2010) Cancer Gene Ther. 17:700.
Breier, G. et al. (1996) Blood 87:630.
Ema, M. et al. (2006) Blood 108:4018.
Oberlin, E. et al. (2010) Blood 116:4444.
Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
Perryn, E.D. et al. (2008) Dev. Biol. 313:545.
Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703.
Taddei, A. et al. (2008) Nat. Cell Biol. 10:923.
Luo, Y. and G.L. Radice (2005) J. Cell Biol. 169:29.
Rudini, N. et al. (2008) EMBO J. 27:993.
Soeki, T. et al. (2004) Circ. J. 68:1.
Sulkowska, M. et al. (2006) Tumori 92:67.
Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.

Long Name

Vascular Endothelium Cadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Alternate Names

7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

Related Research Areas

Citations for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability
Authors: N Gioelli, LJ Neilson, N Wei, G Villari, W Chen, B Kuhle, M Ehling, F Maione, S Willox, S Brundu, D Avanzato, G Koulouras, M Mazzone, E Giraudo, XL Yang, D Valdembri, S Zanivan, G Serini
Nature Communications, 2022-07-20;13(1):4188.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown.
Authors: Zhang P, Feng S, Liu G, Wang H, Zhu H, Ren Q, Bai H, Fu C, Dong C
J Biol Chem, 2015-10-26;291(5):2087-106.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
Authors: Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
Arterioscler. Thromb. Vasc. Biol., 2012-04-26;32(7):1724-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors.
Authors: Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L
Stem Cells, 2007-08-16;25(12):2987-95.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay