Recombinant Human VSTM2A Fc Chimera Avi-tag Protein, CF

Recombinant Human VSTM2A Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is a member of the immunoglobulin superfamily (1). The VSTM family consists of 8 family members, each containing an immunoglobulin V-set (IgV) domain in the extracellular domain (ECD). In addition to the single IgV domain, VSTM2A contains two N-glycosylation sites in the ECD that are critical for its secretion, but not for activity (2). In humans, two isoforms exist due to alternative splicing, varying in the C-terminal residues. The mature ECD of human VSTM2A, isoform 2 shares 80% amino acid sequence identity with mouse and rat VSTM2A isoform 2. VSTM2A is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A promotes regulation and commitment of white and brown preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 2). VSTM2A is highly expressed in the brain but the role of VSTM2A in neuronal and brain development remain uncharacterized (3). VSTM2A is a critical tumor suppressor in colorectal carcinogenesis and a novel antagonist of Wnt signaling receptor LRP6, and might serve as a new prognostic marker for colorectal cancer patients (4). Our in house data show that VSTM2A inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation. Our Avi-tag Biotinylated VSTM2A features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.