Recombinant Mouse AgRP C-Terminal Fragment Protein, CF Summary
Product Specifications
Ser82-Thr131
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
634-AG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: AgRP/ART
Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuroprotein that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) action on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature mouse AgRP is a 111 amino acid (aa) polypeptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Within the C-terminal region, mouse AgRP shares 80% and 90% aa sequence identity with human and rat AgRP, respectively. It also shares 44% aa sequence identity with Agouti. As with Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP, however, is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is up-regulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones via a mechanism that does not involve melanocortin receptors (14).
- Martin, N.M. et al. (2006) Peptides 27:333.
- Fan, W. et al. (1997) Nature 385:165.
- Ollmann, M.M. et al. (1997) Science 278:135.
- Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
- Shutter, J.R. et al. (1997) Genes Dev. 11:593.
- Kiefer, L.L. et al. (1998) Biochemistry 37:991.
- Jackson, P.J. et al. (2002) Biochemistry 41:7565.
- Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
- Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
- Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
- Small, C.K. et al. (2001) Diabetes 50:248.
- Vink, T. et al. (2001) Mol. Psychiatry 6:325.
- Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
- Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.
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Reason for Rating: Easy to be reconstituted.
