Recombinant Mouse Angiopoietin-like 4 Protein, CF
Recombinant Mouse Angiopoietin-like 4 Protein, CF Summary
Product Specifications
Gln24-Ser410, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4880-AN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Angiopoietin-like Protein 4/ANGPTL4
Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature mouse ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with mouse ANGPTL1, 2, 3, 4, 6, and 7. It shares 75% and 97% aa sequence identity with human and rat ANGPTL4, respectively. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).
- Zhu, P. et al. (2012) Biosci. Rep. 32:211.
- Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
- Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
- Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
- Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
- Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
- Cazes, A. et al. (2006) Circ. Res. 99:1207.
- Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
- Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
- Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
- Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
- Hou, M. et al. (2014) PLoS ONE 9:e85808.
- Clement, L.C. et al. (2011) Nat. Med. 17:117.
Citations for Recombinant Mouse Angiopoietin-like 4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Linoleic acid-induced ANGPTL4 inhibits C2C12 skeletal muscle differentiation by suppressing Wnt/beta-catenin
Authors: Y Son, WW Lorenz, CM Paton
The Journal of nutritional biochemistry, 2023-03-23;0(0):109324.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation
Authors: DI Cho, MJ Ahn, HH Cho, M Cho, JH Jun, BG Kang, SY Lim, SJ Yoo, MR Kim, HS Kim, SJ Lee, LT Dat, C Lee, YS Kim, Y Ahn
Experimental & Molecular Medicine, 2023-02-13;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
ANGPTL4 exacerbates pancreatitis by augmenting acinar cell injury through upregulation of C5a
Authors: KH Jung, MK Son, HH Yan, Z Fang, J Kim, SJ Kim, JH Park, JE Lee, YC Yoon, MS Seo, BS Han, S Ko, YJ Suh, JH Lim, DH Lee, Z Teo, JWK Wee, NS Tan, SS Hong
EMBO Mol Med, 2020-07-07;0(0):e11222.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Protective Effects of Angiopoietin-like 4? on the Blood-Brain Barrier in Acute Ischemic Stroke Treated with Thrombolysis in Mice
Authors: B Zhang, X Xu, X Chu, X Yu, Y Zhao
Neurosci. Lett, 2017-03-04;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Angptl4 links alpha-cell proliferation following glucagon receptor inhibition with adipose tissue triglyceride metabolism.
Authors: Ben-Zvi D, Barrandon O, Hadley S, Blum B, Peterson Q, Melton D
Proc Natl Acad Sci U S A, 2015-11-30;112(50):15498-503.
Species: Mouse
Sample Types: In Vivo
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