Recombinant Mouse BCMA/TNFRSF17 Fc Chimera Protein, CF

Catalog #: 593-BC
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
593-BC-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BCMA/TNFRSF17 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED₅₀ for this effect is 0.01-0.03 µg/mL in the presence of 100 ng/mL of rhAPRIL.

Source

Mouse myeloma cell line, NS0-derived mouse BCMA/TNFRSF17 protein

Mouse BCMA (Met1 - Thr49) Accession # NP_035738	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

NP_035738

N-terminal Sequence
Analysis

Met1

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

32 kDa (monomer)

SDS-PAGE

35-40 kDa, reducing conditions

Product Datasheets

593-BC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

593-BC

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

Madry, C. et al. (1998) Int. Immunol. 10:1693.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Marsters, S. et al. (2000) Curr. Biol. 10:785.
Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

Related Research Areas

Citations for Recombinant Mouse BCMA/TNFRSF17 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement.
Authors: Kotani Y, Kunisawa J, Suzuki Y, Sato I, Saito T, Toba M, Kohda N, Kiyono H
PLoS ONE, 2014-03-14;9(3):e91857.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
The Design and Characterization of Receptor-selective APRIL Variants.
Authors: Kimberley, Fiona C, van der Sloot, Almer M, Guadagnoli, Marco, Cameron, Katherin, Schneider, Pascal, Marquart, J Arnoud, Versloot, Miranda, Serrano, Luis, Medema, Jan Paul
J Biol Chem, 2012-09-07;287(44):37434-46.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay
The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
Authors: Suzuki K, Maruya M, Kawamoto S
Immunity, 2010-07-23;33(1):71-83.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay