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Recombinant Mouse beta-Defensin 3 Protein, CF

Catalog #: 5987-BD
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5987-BD-050
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Recombinant Mouse beta-Defensin 3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its anti-microbial activity against E. coli. Ganz, T. et al. (2003) Nat. Rev. Immunol. 3:710. The ED50 for this effect is 4-20 μg/mL.
Source
E. coli-derived mouse beta-Defensin 3 protein
Lys23-Lys63
Accession #
Q9WTL0
N-terminal Sequence
Analysis
Lys23
Predicted Molecular Mass
4.6 kDa
SDS-PAGE
5 kDa, reducing conditions

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5987-BD

Product Datasheet
COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5987-BD

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Defensin 3

beta -defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta -defensins which are distinguished from alpha -defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 41 aa mature mouse BD3 shares 38% and 59% aa sequence identity with human and rat BD3, respectively (2, 3). It shares 14% - 46% aa sequence identity with other mouse beta -defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is up-regulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4 ‑ 6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 and its coreceptor CXCR4 (5, 13) and with HSV glycoprotein B and its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15).

References
  1. Dhople, V. et al. (2006) Biochim. Biophys. Acta. 1758:1499.
  2. Bals, R. et al. (1999) Infect. Immun. 67:3542.
  3. Schibli, D.J. et al. (2002) J. Biol. Chem. 277:8279.
  4. Garcia, J.-R.C. et al. (2001) Cell Tissue Res. 306:257.
  5. Quinones-Mateu, M.E. et al. (2003) AIDS 17:F39.
  6. Sorensen, O.E. et al. (2006) J. Clin. Invest. 116:1878.
  7. Varoga, D. et al. (2005) Arthritis Rheum. 52:1736.
  8. Taggart, C.C. et al. (2003) J. Immunol. 171:931.
  9. Joly, S. et al. (2004) J. Clin. Microbiol. 42:1024.
  10. Chen, X. et al. (2007) Eur. J. Immunol. 37:434.
  11. Kluver, E. et al. (2005) Biochemistry 44:9804.
  12. Wu, Z. et al. (2003) Proc. Natl. Acad. Sci. 100:8880.
  13. Feng, Z. et al. (2006) J. Immunol. 177:782.
  14. Hazrati, E. et al. (2006) J. Immunol. 177:8658.
  15. Leikina, E. et al. (2005) Nat. Immunol. 6:995.
Entrez Gene IDs
55894 (Human)
Alternate Names
BD14; BD3; BD-3; beta-defensin 103; betaDefensin 3; beta-Defensin 3; DEFB103; DEFB103A; DEFB103B; DEFB3; DEFB-3; DEFB3DEFB103A; Defensin, beta 103; defensin, beta 103B; Defensin-like protein; HBD-3; HBD3HBP3

Citation for Recombinant Mouse beta-Defensin 3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Enzymatic modification of lipid A by ArnT protects Bordetella bronchiseptica against cationic peptides and is required for transmission.
    Authors: Rolin O, Muse S, Safi C, Elahi S, Gerdts V, Hittle L, Ernst R, Harvill E, Preston A
    Infect Immun, 2013-10-14;82(2):491-9.
    Species: Bacteria - Bordetella bronchiseptica
    Sample Types: Whole Cells
    Applications: Bioassay

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