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Recombinant Mouse CCL5/RANTES Protein

Catalog #: 478-MR
20 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
478-MR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
478-MR-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (20)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL5/RANTES Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 1.5‑9 ng/mL. Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 30‑100 ng/mL.
Source
E. coli-derived mouse CCL5/RANTES protein
Ser24-Ser91
Accession #
Q5XZF2
N-terminal Sequence
Analysis
Ser24
Predicted Molecular Mass
7.9 kDa

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478-MR (with carrier)

Product Datasheet
COA
SDS

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478-MR/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

478-MR

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

478-MR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75% - 88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

References
  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Heeger, P. et al. (1992) Kidney Int. 41:220.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

Citations for Recombinant Mouse CCL5/RANTES Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. CCL5/CCR5-mediated peripheral inflammation exacerbates blood?brain barrier disruption after intracerebral hemorrhage in mice
    Authors: J Lin, Y Xu, P Guo, YJ Chen, J Zhou, M Xia, B Tan, X Liu, H Feng, Y Chen
    Journal of Translational Medicine, 2023-03-14;21(1):196.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  2. Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
    Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
    PLoS ONE, 2023-01-20;18(1):e0280590.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  3. CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury
    Authors: MH Ho, CH Yen, TH Hsieh, TJ Kao, JY Chiu, YH Chiang, BJ Hoffer, WC Chang, SY Chou
    Redox Biology, 2021-07-17;46(0):102067.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. CCL5 promotion of bioenergy metabolism is crucial for hippocampal synapse complex and memory formation
    Authors: R Ajoy, YC Lo, MH Ho, YY Chen, Y Wang, YH Chen, C Jing-Yuan, CA Changou, YC Hsiung, HM Chen, TH Chang, CY Lee, YH Chiang, WC Chang, B Hoffer, SY Chou
    Molecular Psychiatry, 2021-04-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Neddylation pathway alleviates chronic pancreatitis by reducing HIF1&alpha-CCL5-dependent macrophage infiltration
    Authors: Y Lin, Y Chen, W Feng, R Hua, J Zhang, Y Huo, H Jiang, B Yin, X Yang
    Cell Death & Disease, 2021-03-15;12(3):273.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
    Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
    Front Microbiol, 2019-06-25;10(0):1421.
    Species: Mouse
    Sample Types:
    Applications: Binding Assay
  7. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The HIV co-receptor CCR5 regulates osteoclast function
    Authors: JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura
    Nat Commun, 2017-12-20;8(1):2226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy
    Authors: N Suffee, C Le Visage, H Hlawaty, R Aid-Launai, V Vanneaux, J Larghero, O Haddad, O Oudar, N Charnaux, A Sutton
    Sci Rep, 2017-10-16;7(1):13294.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  10. Ccl5 establishes an autocrine high-grade glioma growth regulatory circuit critical for mesenchymal glioblastoma survival
    Authors: Y Pan, LJ Smithson, Y Ma, D Hambardzum, DH Gutmann
    Oncotarget, 2017-05-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. P2X7 receptor-dependent tuning of gut epithelial responses to infection
    Authors: SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan
    Immunol. Cell Biol, 2016-08-25;95(2):178-188.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Murine IL-17+ Vgamma4 T lymphocytes accumulate in the lungs and play a protective role during severe sepsis.
    Authors: Costa M, de Negreiros C, Bornstein V, Valente R, Mengel J, Henriques M, Benjamim C, Penido C
    BMC Immunol, 2015-06-03;16(0):36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
    Authors: Sottnik J, Dai J, Zhang H, Campbell B, Keller E
    Cancer Res, 2015-04-08;75(11):2151-8.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. KIM-1-mediated phagocytosis reduces acute injury to the kidney.
    Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
    J Clin Invest, 2015-03-09;125(4):1620-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA (Standard)
  15. The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney.
    Authors: Sato S, Zhang X
    Clin Exp Immunol, 2014-07-01;177(1):102-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions.
    Authors: Kimura, Kenichi, Nagano, Masumi, Salazar, Georgina, Yamashita, Toshihar, Tsuboi, Ikki, Mishima, Hajime, Matsushita, Shonosuk, Sato, Fujio, Yamagata, Kenji, Ohneda, Osamu
    Stem Cells Dev, 2013-12-14;23(5):488-501.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5).
    Authors: Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T
    J Bone Miner Res, 2013-10-01;28(10):2070-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011-10-01;179(6):2730-9.
    Applications: ELISA (Standard)
  19. Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis.
    Authors: Binder NB, Niederreiter B, Hoffmann O, Stange R, Pap T, Stulnig TM, Mack M, Erben RG, Smolen JS, Redlich K
    Nat. Med., 2009-03-29;15(4):417-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008-03-26;28(13):3277-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse CCL5/RANTES Protein

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Recombinant Mouse CCL5/RANTES Protein
By Anonymous on 08/12/2019
Application: Immunoassay Standard
Immunoassay Standard Recombinant Mouse CCL5/RANTES Protein 478-MR
Recombinant Mouse CCL5/RANTES Protein
By Anonymous on 11/27/2017
Application: Immunoassay Standard

The recombinant protein was used as an ELISA calibrator employing MAB478 and BAF478 as the matched pair antibodies.

Immunoassay Standard Recombinant Mouse CCL5/RANTES Protein 478-MR