Recombinant Mouse CD200R1L Fc Chimera Protein, CF

Catalog #: 10198-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10198-CD-050

Recombinant Mouse CD200R1L Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD200R1L Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED₅₀ of 0.35-2.8 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CD200R1L protein

Mouse CD200R1L (Ile25-Thr220) Accession # Q6XJV6.1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q6XJV6.1

N-terminal Sequence
Analysis

Ile25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48 kDa

SDS-PAGE

65-85 kDa, under reducing conditions

Product Datasheets

10198-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10198-CD

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED₅₀ of 0.35-2.8 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse CD200R1L Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

Reconstitution Calculator

Background: CD200R1L

CD200 Receptor 2 (CD200R2), or CD200R1L, is a type I transmembrane receptor for the CD200 cell surface glycoprotein. Mature CD200R2 consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a transmembrane segment and a short cytoplasmic domain. The ECD of mouse CD200R2 shares a 76% and 63% amino acid (aa) identity with the rat and human ECD, respectively. CD200R2 is strongly expressed on resting mast cells but deceased upon activation via Fc epsilon R1. It is also observed on monocytes, bone marrow-derived dendritic cells and TH2 cells (1, 2, 4). It has been demonstrated CD200R2 can interact with CD200 (3) and signal through adaptor protein, DAP12, via its lysine residue in the transmembrane region (2). In addition, it has been reported CD200 and CD200R2 interaction alters dendritic cell differentiation and enhances induction of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in mouse transplant model (5).

References