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Recombinant Mouse CD28 Fc Chimera Protein

Catalog #: 483-CD
7 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
483-CD-200/CF

With Carrier

Catalog # Availability Size / Price Qty
483-CD-200
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (7)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD28 Fc Chimera Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.3-1.2 µg/mL when stimulated with 1 µg/mL recombinant human B7-1 Fc Chimera in the presence of PHA.
Source
Mouse myeloma cell line, NS0-derived mouse CD28 protein
Mouse CD28
(Lys21-Lys149)
Accession # P31041		 DMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
P31041
N-terminal Sequence
Analysis
Lys21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa (monomer)
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

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483-CD (with carrier)

Product Datasheet
COA
SDS

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483-CD/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

483-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

483-CD/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD28

CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant co‑stimulatory pathways that regulate T- and B-cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down‑regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up‑regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 ‑ 100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up‑regulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

References
  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.
Entrez Gene IDs
940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)
Alternate Names
CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

Citations for Recombinant Mouse CD28 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models
    Authors: A Hanson, K Elpek, E Duong, L Shallberg, M Fan, C Johnson, M Wallace, GR Mabry, S Sazinsky, L Pepper, CJ Shu, S Sathyanara, S Zuerndorfe, T Simpson, M Gostissa, M Briskin, D Law, J Michaelson, CJ Harvey
    PLoS ONE, 2020-09-24;15(9):e0239595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity.
    Authors: Carmi Y, Spitzer M, Linde I, Burt B, Prestwood T, Perlman N, Davidson M, Kenkel J, Segal E, Pusapati G, Bhattacharya N, Engleman E
    Nature, 2015-04-29;521(7550):99-104.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  3. Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3.
    Authors: Kowalczyk A, D'Souza C, Zhang L
    Eur J Immunol, 2014-01-22;44(4):1143-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cutting edge: chronic inflammatory liver disease in mice expressing a CD28-specific ligand.
    Authors: Corse E, Gottschalk R, Park J, Sepulveda M, Loke P, Sullivan T, Johnson L, Allison J
    J Immunol, 2012-12-17;190(2):526-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Hypoxia-inducible transcription factor-2alpha in endothelial cells regulates tumor neovascularization through activation of ephrin A1.
    Authors: Yamashita T, Ohneda K, Nagano M, Miyoshi C, Kaneko N, Miwa Y, Yamamoto M, Ohneda O, Fujii-Kuriyama Y
    J. Biol. Chem., 2008-04-23;283(27):18926-36.
    Species: Mouse
    Sample Types: Whole Cells
  6. CD86 regulates IgG1 production via a CD19-dependent mechanism.
    Authors: Kin NW, Sanders VM
    J. Immunol., 2007-08-01;179(3):1516-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. BTNL2, a butyrophilin/B7-like molecule, is a negative costimulatory molecule modulated in intestinal inflammation.
    Authors: Arnett HA, Escobar SS, Gonzalez-Suarez E, Budelsky AL, Steffen LA, Boiani N, Zhang M, Siu G, Brewer AW, Viney JL
    J. Immunol., 2007-02-01;178(3):1523-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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