Recombinant Mouse CHL-1/L1CAM-2 Protein, CF

Catalog #: 2147-CH
1 Citation Datasheet / COA / SDS

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2147-CH-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CHL-1/L1CAM-2 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic hippocampal neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived mouse CHL-1/L1CAM-2 protein
Ala25-Gln1043 (Leu227-Gln242 del & Ala243Ser), with a C-terminal 10-His tag

Accession #

BAC30699

N-terminal Sequence
Analysis

Ala25

Predicted Molecular Mass

114 kDa

SDS-PAGE

160-180 kDa, reducing conditions

Product Datasheets

2147-CH

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2147-CH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CHL-1/L1CAM-2

Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1CAM-2), belongs to the L1 subfamily of immunoglobulin (Ig) superfamily cell adhesion molecules, which also includes L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1 - 3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4 - 5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also share a highly conserved cytoplasmic region of approximately 110 amino acid (aa) residues containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p^-syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated increased risk of schizophrenia, has also been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.

References

Moos, M. et al. (1988) Nature 334:701.
Holm, J. et al. (1996) Eur. J. Neusci. 8:1613.
Wei, M. et al. (1998) Hum. Genet. 103:355.
Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813.
Liu, Q. et al. (2000) J. Neurosci. 20:7682.
Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22:7967.
Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.

Long Name

Cell Adhesion Molecule with Homology to L1CAM

Entrez Gene IDs

10752 (Human); 12661 (Mouse)

Alternate Names

CALL; CALLClose homolog of L1; cell adhesion molecule with homology to L1CAM (close homolog of L1); cell adhesion molecule with homology to L1CAM (close homologue of L1); CHL1; CHL-1; FLJ44930; L1CAM-2; L1CAM2L1 cell adhesion molecule 2; MGC132578; neural cell adhesion molecule L1-like protein

Related Research Areas

Citation for Recombinant Mouse CHL-1/L1CAM-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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