Recombinant Mouse CLEC9a Fc Chimera Protein, CF

Catalog #: 6776-CL Datasheet / COA / SDS

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6776-CL-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CLEC9a Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by flow cytometry for its ability to bind a ligand expressed in necrotic EL‑4 mouse lymphoblast cells. When 100 ng of Recombinant Mouse CLEC9a Fc Chimera is added to 5 x 10⁵ EL-4 cells, >50% of necrotic EL-4 cells bind to the protein.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CLEC9a protein

MDP	Mouse IgG_2A (Glu98-Lys330)	IEGR	Mouse CLEC9a (Lys57-Ile264) Accession # NP_001192292
N-terminus			C-terminus

Accession #

NP_001192292

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50.8 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Product Datasheets

6776-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6776-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CLEC9a

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 182 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2‑4). Within the ECD, mouse CLEC9A shares 57% and 80% aa sequence identity with human and rat CLEC9A, respectively. Alternative splicing of mouse CLEC9A generates additional isoforms with insertions in the stalk region or deletions of the transmembrane segment, the CTLD, or a portion of the CTLD (2). Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide‑linked homodimer of approximately 50 kDa N‑glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA‑3⁺ conventional dendritic cells (cDC) and CD16^- monocytes (2‑5). BDCA-3⁺ cDC are analagous to mouse CD8⁺ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2‑5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL‑mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).

References