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Recombinant Mouse DLL4 His-tag Protein

Catalog #: 1389-D4
19 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1389-D4-050/CF

With Carrier

Catalog # Availability Size / Price Qty
1389-D4-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (19)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DLL4 His-tag Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 150-600 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse DLL4 protein
Ser28-Pro525, with a C-terminal 10-His tag
Accession #
BAB18580
N-terminal Sequence
Analysis
Ser28
Predicted Molecular Mass
55.6 kDa
SDS-PAGE
75-80 kDa, reducing conditions

Product Datasheets

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1389-D4 (with carrier)

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1389-D4/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1389-D4

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG3350 with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1389-D4/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG3350.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2 - 4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

References
  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name
Delta-like 4
Entrez Gene IDs
54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names
Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4

Citations for Recombinant Mouse DLL4 His-tag Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Identification of Key Determinants of Cerebral Malaria Development and Inhibition Pathways
    Authors: SJ Cha, X Yu, BD Gregory, YS Lee, T Ishino, RO Opoka, CC John, M Jacobs-Lor
    MBio, 2022-01-25;0(0):e0370821.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Protective effect of Soluble Epoxide Hydrolase Inhibition in Retinal Vasculopathy associated with Polycystic Kidney Disease
    Authors: J Lin, J Hu, A Schlottere, J Wang, M Kolibabka, K Awwad, N Dietrich, K Breitschop, P Wohlfart, A Kannt, K Lorenz, Y Feng, R Popp, S Hoffmann, I Fleming, HP Hammes
    Theranostics, 2020-06-22;10(17):7857-7871.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis
    Authors: R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu
    Nat Commun, 2020-01-23;11(1):460.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Notch1 signaling in NOTCH1-mutated mantle cell lymphoma depends on Delta-Like ligand 4 and is a potential target for specific antibody therapy
    Authors: E Silkensted, F Arenas, B Colom-Sanm, S Xargay-Tor, M Higashi, A Giró, V Rodriguez, P Fuentes, WE Aulitzky, H van der Ku, S Beà, ML Toribio, E Campo, M López-Guer, D Colomer
    J. Exp. Clin. Cancer Res., 2019-11-01;38(1):446.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells
    Authors: MFM Gerli, LA Moyle, S Benedetti, G Ferrari, E Ucuncu, M Ragazzi, C Constantin, I Louca, H Sakai, P Ala, P De Coppi, S Tajbakhsh, G Cossu, FS Tedesco
    Stem Cell Reports, 2019-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Impaired Notch Signaling Leads to a Decrease in p53 Activity and Mitotic Catastrophe in Aged Muscle Stem Cells
    Authors: L Liu, GW Charville, TH Cheung, B Yoo, PJ Santos, M Schroeder, TA Rando
    Cell Stem Cell, 2018-09-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection
    Authors: HA Ting, D de Almeida, AJ Rasky, CA Malinczak, IP Maillard, MA Schaller, NW Lukacs
    Mucosal Immunol, 2018-07-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Asiatic acid attenuates lipopolysaccharide-induced injury by suppressing activation of the Notch signaling pathway
    Authors: X Yuyun, C Xi, Y Qing, X Lin, R Ke, S Bingwei
    Oncotarget, 2018-01-23;9(19):15036-15046.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development
    Authors: T Kostrzewsk, AJ Borg, Y Meng, I Filipovic, V Male, A Wack, PA DiMaggio, HJM Brady
    J. Immunol., 2018-01-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury
    Authors: H Suzuki, CS Ahuja, RP Salewski, L Li, K Satkunendr, N Nagoshi, S Shibata, MG Fehlings
    PLoS ONE, 2017-08-03;12(8):e0182339.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow.
    Authors: Yu V, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang Y, Lymperi S, Ferraro F, Raaijmakers M, Wu J, Zhou L, Rajagopal J, Kronenberg H, Baron R, Scadden D
    J Exp Med, 2015-04-27;212(5):759-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. Tumor-infiltrating myeloid cells activate Dll4/Notch/TGF-beta signaling to drive malignant progression.
    Authors: Ohnuki H, Jiang K, Wang D, Salvucci O, Kwak H, Sanchez-Martin D, Maric D, Tosato G
    Cancer Res, 2014-02-11;74(7):2038-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Muller glia cells regulate Notch signaling and retinal angiogenesis via the generation of 19,20-dihydroxydocosapentaenoic acid.
    Authors: Hu J, Popp R, Fromel T, Ehling M, Awwad K, Adams R, Hammes H, Fleming I
    J Exp Med, 2014-01-20;211(2):281-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Dll4 and PDGF-BB convert committed skeletal myoblasts to pericytes without erasing their myogenic memory.
    Authors: Cappellari O, Benedetti S, Innocenzi A, Tedesco F, Moreno-Fortuny A, Ugarte G, Lampugnani M, Messina G, Cossu G
    Dev Cell, 2013-03-07;24(6):586-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
    Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
    Nature, 2012-03-18;484(7392):110-4.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  17. Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma.
    Authors: Haller BK, Brave A, Wallgard E, Roswall P, Sunkari VG, Mattson U, Hallengard D, Catrina SB, Hellstrom M, Pietras K
    Oncogene, 2010-05-24;29(30):4276-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Attenuation of Notch signaling promotes the differentiation of neural progenitors into neurons in the hippocampal CA1 region after ischemic injury.
    Authors: Oya S, Yoshikawa G, Takai K, Tanaka JI, Higashiyama S, Saito N, Kirino T, Kawahara N
    Neuroscience, 2008-10-31;158(2):683-92.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  19. Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells.
    Authors: Carlson ME, Hsu M, Conboy IM
    Nature, 2008-06-15;454(7203):528-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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