Recombinant Mouse DR3/TNFRSF25 Fc Chimera Protein, CF

Catalog #: 2437-D3 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2437-D3-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DR3/TNFRSF25 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse TL1A/TNFSF15 (Catalog # 1896-TL/CF) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse DR3/TNFRSF25 Fc Chimera that produces 50% of the optimal binding response is approximately 0.75-4 μg/mL

Source

Mouse myeloma cell line, NS0-derived mouse DR3/TNFRSF25 protein

Mouse DR3 (Gln31-Phe199) Accession # AAK11256	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAK11256

N-terminal Sequence
Analysis

No results obtained: Gln31 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

44.6 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Product Datasheets

2437-D3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2437-D3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: DR3/TNFRSF25

Death receptor 3 (DR3), also known as TNFRSF25, LARD, WSL-1, APO3, TRAMP, and TR3, is a 55 kDa TNF receptor superfamily protein that is predominantly expressed by lymphocytes. TNF receptor superfamily members have varying numbers of extracellular cysteine-rich domains (CRDs) with conserved cysteine spacing (1, 2). DR3 contains four CRDs and one cytoplasmic death domain (3, 4). Alternative splicing of mouse DR3 generates an isoform that lacks the fourth CRD and a secreted isoform that consisits of only the extracellular domain (ECD) (3). Human DR3 exists in at least eleven alternate splice forms (5). Within the ECD, mouse and human DR3 share 59% amino acid (aa) sequence identity. DR3 shares 20%-28% aa sequence identity with the ECD of death domain receptors DR5, DR6, EDAR, Fas, NGF R, and TNF RI. Naïve B and T cells preferentially express truncated soluble isoforms of DR3, whereas stimulated lymphocytes preferentially express transmembrane DR3 (5). TL1A/TNFSF15, a high affinity DR3 ligand which also exists in membrane bound and soluble forms, is expressed by activated endothelial cells and T cells (6, 7). TL1A additionally binds to DcR3/TNFRSF6B, a soluble decoy receptor that interferes with DR3 activation (8). DR3 signaling triggers either apoptosis or NF kappa B-induced anti-apoptotic effects depending on the cellular setting (9). Apoptosis is partially impaired during negative selection of thymocytes in DR3-null mice (10). TL1A interactions with DR3 augment T cell proliferation and proinflammatory cytokine secretion (6, 7, 11, 12). DR3 is upregulated by inflammatory stimulation of CCR9⁺ T cells, a T cell subset important in mucosal immunity (11). T cell and macrophage DR3 expression is prominent in several inflammatory disorders such as Crohn’s disease, inflammatory bowel disease, and atherosclerosis (7, 11-15). DR3 activation on IFN-gamma treated THP-1 cells induces the production of TNF-alpha, CXCL8, CCL2, MMP-1, -9, and -13 (14, 15).

References

Fas, S.C. et al. (2006) Curr. Dir. Autoimmun. 9:1.
Aggarwal, B.B. (2003) Nat. Rev. Immunol. 3:745.
Wang, E.C.Y. et al. (2001) Immunogenetics 53:59.
Borysenko, C.W. et al. (2005) Biochem. Biophys. Res. Commun. 328:794.
Screaton, G.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:4615.
Migone, T.S. et al. (2002) Immunity 16:479.
Prehn, J.L. et al. (2004) Clin. Immunol. 112:66.
Yang, C.-R. et al. (2004) Cancer Res. 64:1122.
Wen, L. et al. (2003) J. Biol. Chem. 278:39251.
Wang, E.C.Y. et al. (2001) Mol. Cell. Biol. 21:3451.
Papadakis, K.A. et al. (2005) J. Immunol. 174:4985.
Papadakis, K.A. et al. (2004) J. Immunol. 172:7002.
Bamias, G. et al. (2003) J. Immunol. 171:4868.
Kim, S.-H. et al. (2001) Jpn. Circ. J. 65:136.
Kang, Y.-J. et al. (2005) Cytokine 29:229.

Long Name

Death Receptor 3

Entrez Gene IDs

8718 (Human); 85030 (Mouse)

Alternate Names

APO3; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; DR3; DR3member 12 (translocatingchain-association membrane protein); LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF25; TRAMP; tumor necrosis factor receptor superfamily, member 25; WSL; WSL1; WSL-1