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Recombinant Mouse Ephrin-A5 Fc Chimera Protein, CF

Catalog #: 7396-EA
6 Citations Datasheet / COA / SDS
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7396-EA-050
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Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Ephrin-A5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED50 for this effect is 2.5-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-A5 protein
Mouse Ephrin-A5
(Met1-Asn203)
Accession # NP_997537		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
NP_997537
N-terminal Sequence
Analysis
No results obtained: Gln21 predicted, sequencing might be blocked
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48.4 kDa (monomer)
SDS-PAGE
57-60 kDa, reducing conditions

Product Datasheets

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7396-EA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7396-EA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A5

Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature mouse Ephrin‑A5 shares 98.9% and 99.5% aa sequence identity with human and rat Ephrin‑A5 (4). Alternate splicing of mouse Ephrin‑A5 generates a short isoform that lacks 27 amino acids in the juxtamembrane region. The short isoform retains the ability to bind EphA3 and inhibit neurite extension (5). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (6, 7). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (7‑11). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 12, 13). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (14). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (10, 15, 16). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (15). Ephrin‑A5 functions as a tumor suppressor and its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (17). Ephrin A5 is also down‑regulated in prostate cancer (18). Ephrin‑A5 is expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (19).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
  4. Flenniken, A.M. et al. (1996) Dev. Biol. 179:382.
  5. Lai, K.-O. et al. (1999) FEBS Lett. 458:265.
  6. Deschamps, C. et al. (2010) BMC Neurosci. 11:105.
  7. Cooper, M.A. et al. (2009) Dev. Neurobiol. 69:36.
  8. Frisen, J. et al. (1998) Neuron 20:235.
  9. Zimmer, G. et al. (2008) Eur. J. Neurosci. 28:62.
  10. Otal, R. et al. (2006) Neuroscience 141:109.
  11. Eberhart, J. et al. (2004) J. Neurosci. 24:1070.
  12. Munoz, L.M. et al. (2005) Dev. Biol. 283:397.
  13. Meier, C. et al. (2011) PLoS ONE 6:e26089.
  14. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  15. Hara, Y. et al. (2010) Stem Cells 28:974.
  16. Akaneya, Y. et al. (2010) PLoS ONE 5:e12486.
  17. Li, J.-J. et al. (2009) Oncogene 28:1759.
  18. Gustavsson, H. et al. (2008) Prostate 68:161.
  19. Swartz, M.E. et al. (2001) Development 128:4669.
Entrez Gene IDs
1946 (Human); 13640 (Mouse)
Alternate Names
AF1; AL-1; EFL-5; EFNA5; EPH-related receptor tyrosine kinase ligand 7; EphrinA5; Ephrin-A5; EPLG7; GLC1M; LERK-7; LERK7EFL5; RAGS

Citations for Recombinant Mouse Ephrin-A5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation
    Authors: O Ros, Y Zagar, S Ribes, S Baudet, K Loulier, S Couvet, D Ladarre, A Aghaie, A Louail, C Petit, Y Mechulam, Z Lenkei, X Nicol
    Cell Rep, 2019-06-25;27(13):4003-4012.e6.
    Species: Mouse
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  2. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon
    Authors: YH Liu, JW Tsai, JL Chen, WS Yang, PC Chang, PL Cheng, DL Turner, Y Yanagawa, TW Wang, JY Yu
    Sci Rep, 2017-03-09;7(0):42895.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
    Mol Med Rep, 2016-12-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Thalamic afferents influence cortical progenitors via ephrin A5-EphA4 interactions
    Authors: K Gerstmann, D Pensold, J Symmank, M Khundadze, CA Hübner, J Bolz, G Zimmer
    Development, 2014-12-05;142(1):140-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Reelin induces EphB activation.
    Authors: Bouche E, Romero-Ortega M, Henkemeyer M, Catchpole T, Leemhuis J, Frotscher M, May P, Herz J, Bock H
    Cell Res, 2013-01-15;23(4):473-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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