Recombinant Mouse FAM3C Protein, CF

Catalog #: 2868-FM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2868-FM-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FAM3C Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse FAM3C protein
Gln25-Asp227, with a C-terminal 6-His tag

Accession #

Q91VU0

N-terminal Sequence
Analysis

No results obtained: Gln25 predicted, sequencing might be blocked

Predicted Molecular Mass

23 kDa

SDS-PAGE

23-25 kDa, reducing conditions

Product Datasheets

2868-FM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2868-FM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FAM3C

Family with sequence similarity 3, member C (FAM3C), also known as Interleukin-Like EMT Inducer (ILEI), is an approximately 25 kDa secreted protein in the FAM group of molecules (1, 2). Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. Mature mouse FAM3C shares 94% and 97% amino acid sequence identity with human and rat FAM3C, respectively. It is widely expressed, notably in the nonsensory epithelium of auditory semicircular canals (1) and in the photoreceptor layer of the retina (3). FAM3C is up‑regulated and shows altered intracellular localization in various tumors (4, 5). Its translation is normally silenced by the binding of hnRNP E1 to the FAM3C mRNA, but this inhibition is removed following cellular exposure to TGF-beta (6, 7). FAM3C, like TGF-beta, promotes epithelial-mesenchymal transition (EMT), tumor growth, and metastasis (4-6). FAM3C cooperates with oncogenic Ras to promote the up‑regulation of PDGF-C and PDGF R beta, leading to increased PDGF receptor signaling (5). R&D Systems in-house testing indicates that FAM3C supports the adhesion of Caki cells.

References