Recombinant Mouse GDF-8 Propeptide Fc Chimera Protein

Catalog #: 6986-PG Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
6986-PG-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
6986-PG-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GDF-8 Propeptide Fc Chimera Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Thies, R.S. et al. (2001) Growth Factors 18:251. The ED₅₀ for this effect is 0.03-0.15 μg/mL in the presence of 40 ng/mL of Recombinant Mouse GDF‑8/Myostatin.

Source

Mouse myeloma cell line, NS0-derived mouse GDF-8/Myostatin protein

Mouse GDF-8 Propeptide (Asn25-Arg267) with a substitution Ser265Arg Accession # NP_034964	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_034964

N-terminal Sequence
Analysis

Asn25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

54.9 kDa (monomer)

SDS-PAGE

66 kDa, reducing conditions

Product Datasheets

6986-PG (with carrier)

Product Datasheet

COA

SDS

6986-PG/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6986-PG

Formulation	Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CHAPS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6986-PG/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CHAPS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF‑8), also known as Myostatin, is a secreted TGF‑ beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF‑8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF‑8 shares 96% and 99% aa sequence identity with human and rat GDF‑8, respectively. GDF‑8 is secreted as a preproprotein that is cleaved by BMP‑1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide‑linked dimer of the mature protein and two noncovalently‑associated molecules of the propeptide (2, 6). The GDF‑8 propeptide functions as an inhibitor of mature GDF‑8, and GDF‑8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP‑1 (6‑11). The uncleaved GDF‑8 proprotein binds Latent TGF‑ beta binding protein 3 (LTBP3) which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF‑8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK‑4 or TGF‑ beta RI/ALK‑5 to induce signaling (13). GDF‑8 additionally inhibits adipogenic differentiation of human bone marrow‑derived mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF‑8 or in vivo administration of the GDF‑8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

References