Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein, CF

Catalog #: 9906-S4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9906-S4-050

Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera, immobilized at 0.5-1 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Source

Mouse myeloma cell line, NS0-derived mouse IGSF4C/SynCAM4 protein

Mouse IGSF4C (Gln25-Ala324) Accession # Q8R464	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8R464

N-terminal Sequence
Analysis

No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Glu26.

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

60 kDa

SDS-PAGE

81-93 kDa, reducing conditions

Product Datasheets

9906-S4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9906-S4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera (Catalog # 9906-S4) Induces Cortical Neurite Outgrowth. A) Untreated E16-18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-18 embryonic rat cortical neurons treated with 0.5 μg/mL of Recombinant Mouse IGSF4C/SynCAM4 Fc Chimera.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IGSF4C was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 81-93 kDa and 160-190 kDa, respectively.

Reconstitution Calculator

Background: IGSF4C/SynCAM4

IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1-5). Each contain one V-type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca⁺⁺-independent homophilic and heterophilic interactions. The 388 a mouse IGSF4C contains a 24 aa signal sequence, a 300 aa ECD that shares 99% amino acid (aa) identity with rat and human IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48-67 kDa, probably due to differences in glycosylation (2, 5, 6). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6-8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 7). IGSF4C is also proposed as a tumor suppressor that is down-regulated in many prostate cancers and gliomas (1, 5).

References

Fukuhara, H. et al. (2001) Oncogene 20:5401.
Biederer, T. et al. (2006) Genomics 87:139.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Thomas, L. A. et al. (2008) J. Comp. Neurol. 510:47.
Williams, Y. N. et al. (2005) Oncogene 25:1446.
Maurel, P. et al. (2007) J. Cell Biol. 178:861.
Fogel, A. I. et al. (2007) J. Neurosci. 27:12516.
Spiegel, I. et al. (2007) Nat. Neurosci. 10:861.

Long Name

Immunoglobulin Superfamily, Member 4B

Entrez Gene IDs

199731 (Human); 260299 (Mouse)

Alternate Names

CADM4; cell adhesion molecule 4; IGSF4C; IGSF4Cnectin-like 4; Immunoglobulin superfamily member 4C; immunoglobulin superfamily, member 4C; Necl-4; NECL4synCAM4; Nectin-like protein 4; SynCAM4; TSLC1-like 2; TSLC1-like protein 2; TSLL2; TSLL2SynCAM4