Recombinant Mouse IL-17B Protein

Catalog #: 1709-ML
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1709-ML-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1709-ML-025

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (1)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-17B Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. Tang, Y. et al. (2011) Clin. Exp. Immunol. 166:281. The ED₅₀ for this effect is 0.4-2 μg/mL.

Source

E. coli-derived mouse IL-17B protein
His21-Phe180, with an N-terminal Met

Accession #

Q9QXT6

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

18.2 kDa (monomer)

Product Datasheets

1709-ML (with carrier)

Product Datasheet

COA

SDS

1709-ML/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1709-ML

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1709-ML/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-17B

Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17B shares 90% and 98% amino acid sequence identity with human and rat IL-17B, respectively (3). IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine (3-5). In the skeletal system, IL-17B is expressed in bone collars surrounding primary ossification centers as well as in epiphyseal plate chondrocytes during development and after bone fracture (6, 7). IL-17B is up-regulated in arthritic cartilage where it exacerbates the severity of disease (8). It is also up-regulated in aggressive breast cancer cell lines, and its exaggerated signaling through IL-17RB promotes tumorigenicity (9). It inhibits the adhesion of vascular endothelial cells to Matrigel and enhances their migration (10)

References

Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89.
You, Z. et al. (2005) Biochem. Biophys. Res. Commun. 326:624.
Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
Huang, C.-K. et al. (2013) Oncogene July 15 Epub.
Sanders, A.J. et al. (2010) J. Oncol. 2010:817375.

Long Name

Interleukin 17B

Entrez Gene IDs

27190 (Human); 56069 (Mouse)

Alternate Names

Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20

Related Research Areas

Citation for Recombinant Mouse IL-17B Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Interleukin-17B Antagonizes Interleukin-25-Mediated Mucosal Inflammation.
Authors: Reynolds J, Lee Y, Shi Y, Wang X, Angkasekwinai P, Nallaparaju K, Flaherty S, Chang S, Watarai H, Dong C
Immunity, 2015-04-14;42(4):692-703.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay