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Recombinant Mouse IL-21R Fc Chimera Protein, CF

Catalog #: 596-MR
30 Citations Datasheet / COA / SDS
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596-MR-100
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Citations (30)
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Recombinant Mouse IL-21R Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 75-500 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-21 R protein
Mouse IL-21 R Subunit
(Cys20 - Pro236)
Accession # Q9JHX3		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
Q9JHX3
N-terminal Sequence
Analysis
Cys20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.5 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions

Product Datasheets

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596-MR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

596-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21R

IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1 ‑ 5). Complex formation between IL-21 R and the common gamma  chain ( gamma c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL‑21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3 ‑ 5). Both IL‑21 and IL‑4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL‑21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10).  IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8+ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL‑21 R expression is often up‑regulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

References
  1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
  2. Konforte, D. et al. (2009) J. Immunol. 182:1791.
  3. Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Asao, H. et al. (2001) J. Immunol. 167:1.
  7. Habib, T. et al. (2002) Biochemistry 41:8725.
  8. Ozaki, K. et al. (2002) Science 298:1630.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. King, I.L. et al. (2010) J. Immunol. 185:6138.
  11. Kasaian, M.T. et al. (2002) Immunity 16:559.
  12. Frohlich, A. et al. (2009 Science 324:1576.
  13. Tortola, L. et al. (2010) Blood 116:5200.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.
Long Name
Interleukin 21 Receptor
Entrez Gene IDs
50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names
CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

Citations for Recombinant Mouse IL-21R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Maternal schistosomiasis impairs offspring Interleukin-4 production and B cell expansion
    Authors: D Cortés-Sel, L Gibbs, A Ready, HA Ekiz, R O'Connell, B Rajwa, KC Fairfax
    PloS Pathogens, 2021-02-01;17(2):e1009260.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. ICOS signaling promotes a secondary humoral response after re-challenge with Plasmodium�chabaudi chabaudi AS
    Authors: LE Latham, DJ Wikenheise, JS Stumhofer
    PLoS Pathog., 2020-04-29;16(4):e1008527.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor
    Authors: L Wang, E Shen, L Luo, H Rabe, Q Wang, J Yin, X Yang, W Liu, JM Sido, H Nakagawa, L Ao, HJ Kim, H Cantor, JW Leavenwort
    Cell Rep, 2019-11-12;29(7):1848-1861.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population
    Authors: MD Powell, KA Read, BK Sreekumar, DM Jones, KJ Oestreich
    Sci Rep, 2019-09-30;9(1):13991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Interleukin-21-mediated suppression of the Pax3-Id3 pathway exacerbates the development of Sj�gren's syndrome via follicular helper T cells
    Authors: JS Park, SM Kim, J Choi, KA Jung, SH Hwang, S Yang, SK Kwok, ML Cho, SH Park
    Cytokine, 2019-09-03;125(0):154834.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. IL-4 Promotes stromal cell expansion and Is critical for development Of A Type-2, but not a type 1 immune response
    Authors: D Cortes-Sel, A Ready, L Gibbs, B Rajwa, KC Fairfax
    Eur. J. Immunol., 2019-01-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection
    Authors: Q Cheng, J Liu, Y Pei, Y Zhang, D Zhou, W Pan, J Zhang
    PLoS Pathog., 2018-11-21;14(11):e1007440.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Suppression of Th1 Priming by TLR2 Agonists during Cutaneous Immunization Is Mediated by Recruited CCR2+ Monocytes
    Authors: CT Johndrow, MF Goldberg, AJ Johnson, TW Ng, S Kunnath-Ve, G Lauvau, DH Kaplan, GH Gossel, UD Kadolsky, AJ Yates, J Chan, WR Jacobs, SA Porcelli
    J. Immunol., 2018-11-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease
    Authors: KM Valentine, D Davini, TJ Lawrence, GN Mullins, M Manansala, M Al-Kuhlani, JM Pinney, JK Davis, AE Beaudin, SS Sindi, DM Gravano, KK Hoyer
    J. Immunol., 2018-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus
    Authors: JY Choi, A Seth, M Kashgarian, S Terrillon, E Fung, L Huang, LC Wang, J Craft
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Antigen-presenting cell-derived IL-6 restricts the expression of GATA3 and IL-4 by follicular helper T cells
    J Leukoc Biol, 2016-07-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Splenic Long-Lived Plasma Cells Promote the Development of Follicular Helper T Cells during Autoimmune Responses.
    Authors: Jang E, Cho W, Oh Y, Cho M, Kim J, Paik D, Youn J
    J Immunol, 2016-01-04;196(3):1026-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The Costimulatory Molecule ICOS Regulates Host Th1 and Follicular Th Cell Differentiation in Response to Plasmodium chabaudi chabaudi AS Infection.
    Authors: Wikenheiser D, Ghosh D, Kennedy B, Stumhofer J
    J Immunol, 2015-12-14;196(2):778-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Somatic mutations and affinity maturation are impaired by excessive numbers of T follicular helper cells and restored by Treg cells or memory T cells.
    Authors: Preite S, Baumjohann D, Foglierini M, Basso C, Ronchi F, Rodriguez B, Corti D, Lanzavecchia A, Sallusto F
    Eur J Immunol, 2015-10-01;45(11):3010-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. B Cell-Specific MHC Class II Deletion Reveals Multiple Nonredundant Roles for B Cell Antigen Presentation in Murine Lupus.
    Authors: Giles J, Kashgarian M, Koni P, Shlomchik M
    J Immunol, 2015-08-12;195(6):2571-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Local triggering of the ICOS coreceptor by CD11c(+) myeloid cells drives organ inflammation in lupus.
    Authors: Teichmann L, Cullen J, Kashgarian M, Dong C, Craft J, Shlomchik M
    Immunity, 2015-03-17;42(3):552-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells.
    Authors: Kim Y, Lim H, Jung H, Wetsel R, Chung Y
    PLoS ONE, 2015-03-13;10(3):e0120294.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Disruption of IL-21 signaling affects T cell-B cell interactions and abrogates protective humoral immunity to malaria.
    Authors: Perez-Mazliah D, Ng D, Freitas do Rosario A, McLaughlin S, Mastelic-Gavillet B, Sodenkamp J, Kushinga G, Langhorne J
    PLoS Pathog, 2015-03-12;11(3):e1004715.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  19. IL-4-secreting secondary T follicular helper (Tfh) cells arise from memory T cells, not persisting Tfh cells, through a B cell-dependent mechanism.
    Authors: Fairfax K, Everts B, Amiel E, Smith A, Schramm G, Haas H, Randolph G, Taylor J, Pearce E
    J Immunol, 2015-02-23;194(7):2999-3010.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. ICOS maintains the T follicular helper cell phenotype by down-regulating Kruppel-like factor 2.
    Authors: Weber J, Fuhrmann F, Feist R, Lahmann A, Al Baz M, Gentz L, Vu Van D, Mages H, Haftmann C, Riedel R, Grun J, Schuh W, Kroczek R, Radbruch A, Mashreghi M, Hutloff A
    J Exp Med, 2015-02-02;212(2):217-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.
    Authors: Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E
    J Immunol, 2014-07-28;193(5):2306-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. CD73 expression is dynamically regulated in the germinal center and bone marrow plasma cells are diminished in its absence.
    Authors: Conter, Laura J, Song, Eunice, Shlomchik, Mark J, Tomayko, Mary M
    PLoS ONE, 2014-03-24;9(3):e92009.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells.
    Authors: Vogelzang, Alexis, McGuire, Helen M, Liu, Sue M, Gloss, Brian, Mercado, Karessa, Earls, Peter, Dinger, Marcel E, Batten, Marcel, Sprent, Jonathan, King, Cecile
    J Immunol, 2014-01-20;192(4):1404-14.
    Species: Mouse
    Sample Types: Protein, Whole Cells
    Applications: ELISA, Flow Cytometry
  24. Excess IL-1 signaling enhances the development of Th17 cells by downregulating TGF-beta-induced Foxp3 expression.
    Authors: Ikeda S, Saijo S, Murayama M, Shimizu K, Akitsu A, Iwakura Y
    J Immunol, 2014-01-15;192(4):1449-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Myor/ABF-1 mRNA [corrected] Expression Marks Follicular Helper T Cells but Is Dispensable for Tfh Cell Differentiation and Function In Vivo.
    Authors: Debuisson D, Mari N, Denanglaire S, Leo O, Andris F
    PLoS ONE, 2013-12-26;8(12):e84415.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. OX40 facilitates control of a persistent virus infection.
    Authors: Boettler, Tobias, Moeckel, Friedric, Cheng, Yang, Heeg, Maximili, Salek-Ardakani, Shahram, Crotty, Shane, Croft, Michael, von Herrath, Matthias
    PLoS Pathog, 2012-09-06;8(9):e1002913.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. T-follicular helper cells survive as long-term memory cells.
    Eur. J. Immunol., 2012-08-01;42(8):1981-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity.
    Authors: McGuire HM, Vogelzang A, Ma CS, Hughes WE, Silveira PA, Tangye SG, Christ D, Fulcher D, Falcone M, King C
    Immunity, 2011-04-22;34(4):602-15.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Flow Cytometry, In Vivo
  29. B and T lymphocyte attenuator suppresses IL-21 production from follicular Th cells and subsequent humoral immune responses.
    Authors: Kashiwakuma D, Suto A, Hiramatsu Y
    J. Immunol., 2010-07-26;185(5):2730-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. In vivo regulation of Bcl6 and T follicular helper cell development.
    Authors: Poholek AC, Hansen K, Hernandez SG
    J. Immunol., 2010-06-02;185(1):313-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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