Recombinant Mouse L-Selectin/CD62L Fc Chimera Protein, CF

Catalog #: 576-LS
4 Citations Datasheet / COA / SDS

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576-LS-100

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse L-Selectin/CD62L Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells.

When 5 x 10⁴ cells/well are added to Recombinant Mouse L‑Selectin/CD62L Fc Chimera coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.3‑1.2 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse L-Selectin/CD62L protein

Mouse L-Selectin (Trp39 - Asn332) Accession # Q3UV83	IEGRMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q3UV83

N-terminal Sequence
Analysis

Trp39

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

60.5 kDa (monomer)

SDS-PAGE

150 kDa, reducing conditions

Product Datasheets

576-LS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

576-LS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 0.1 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: L-Selectin/CD62L

L-Selectin (also known as Leukocyte Selectin, LAM‑1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, and CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L-Selectin share 76% amino acid sequence homology.

L-Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble L-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Alternate Names

CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

Related Research Areas

Citations for Recombinant Mouse L-Selectin/CD62L Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Platelets Guide Leukocytes to Their Sites of Extravasation
PLoS Biol, 2016-05-06;14(5):e1002459.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
Nat Commun, 2015-03-31;6(0):6387.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation.
Authors: Shigeta A, Matsumoto M, Tedder TF, Lowe JB, Miyasaka M, Hirata T
Blood, 2008-09-25;112(13):4915-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression.
Authors: Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS
J. Allergy Clin. Immunol., 2007-10-01;121(1):148-157.e3.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry