Recombinant Mouse LIFR alpha Protein, CF Summary
Product Specifications
Leu44-Ser828, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5990-LR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: LIFR alpha
Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12 - 14).
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- Gearing, D.P. et al. (1991) EMBO J. 10:2839.
- Tomida, M. et al. (1994) J. Biochem. 115:557.
- Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
- Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
- Gearing, D.P. et al. (1992) Science 255:1434.
- Giese, B. et al. (2005) J. Cell Sci. 118:5129.
- Ip, N.Y. et al. (1992) Cell 69:1121.
- Davis, S. et al. (1993) Science 260:1805.
- Metcalf, D (2003) Stem Cells 21:5.
- Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
- Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
- Stewart, C.L. et al. (1992) Nature 359:76.
- Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.
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