Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein, CF

Catalog #: 9320-T4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9320-T4-050

Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) is coated at 2 µg/mL, Recombinant Mouse LILRA6/CD85b Fc Chimera binds with an ED₅₀ = 5-30 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse LILRA6/CD85b protein

Mouse LILRA6/CD85b/ILT8 (Gly24-Asn629) Accession # NP_035220	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_035220

N-terminal Sequence
Analysis

Gly24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

95 kDa

SDS-PAGE

97-118 kDa, reducing conditions

Product Datasheets

9320-T4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9320-T4

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) is coated at 2 µg/mL, Recombinant Mouse LILRA6/CD85b/ILT8 Fc Chimera (Catalog # 9320-T4) binds with an ED₅₀ = 5-30 ng/mL.

Reconstitution Calculator

Background: LILRA6/CD85b

LILRA6, also known as ILT8, CD85b, and PIR-A3 (in mouse), is a transmembrane molecule that belongs to the LILR family of immune regulatory proteins (1, 2). LILRA family members augment immune responses, while LILRB proteins inhibit signaling events (3). The extracellular region (ECD) of mouse LILRA6/PIR-A3 contains 6 immunoglobulin-like domains in its extracellular region, although the human protein contains four Ig-like domains. Within common regions of their ECDs, human LILRA6 shares 53% amino acid sequence identity with mouse PIR-A3. It contains a positively charged arginine residue in the transmembrane segment which mediates association with the signaling protein Fc epsilon RI gamma (4). LILRA6 is primarily expressed on monocytes (5). Its gene copy number is highly variable and is positively associated with the development of atopic dermatitis (2, 6). Allelic polymorphisms are differentially able to mediate immune cell detection of necrotic cells (7, 8). R&D Systems in-house testing indicates that LILRA6 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (9).

References