Recombinant Mouse MAGP-2/MFAP5 Protein, CF

Catalog #: 7345-MG Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7345-MG-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MAGP-2/MFAP5 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells.

When 2 x 10⁴ cells/well are added to Recombinant Mouse MAGP-2/MFAP5 coated plates (10 μg/mL with 100 μL/well), approximately 45-70% will adhere after 1 hour at 37 °C.

Optimal dilutions should be determinded by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse MAGP-2/MFAP5 protein
Gln29-Leu164 with a C-terminal 6-His tag

Accession #

AAH25131

N-terminal Sequence
Analysis

Gln29 predicted, sequencing might be blocked, no result obtained

Predicted Molecular Mass

16.5 kDa

SDS-PAGE

22-30 kDa, reducing conditions

Product Datasheets

7345-MG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7345-MG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MAGP-2/MFAP5

MAGP-2 (Microfibril-associated glycoprotein 2), also called MFAP5 (microfibrillar associated protein 5) is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins (1). Mouse MAGP-2 cDNA encodes 164 amino acids (aa) including a 28 aa signal sequence, an RDG motif (aa 30 ‑ 32; absent in MAGP-1) and a central cysteine‑rich binding domain (aa 76 ‑ 133) (1 ‑ 3). Mature mouse MAGP-2 shares 86%, 96%, 84%, 85% and 85% aa identity with human, rat, bovine, porcine and canine MAGP-2, respectively. Both MAGP-1 and MAGP-2 bind and covalently crosslink Fibrillin-1 and -2 through the Cys-rich binding domain. MAGP-2, however, has a more limited distribution and binds a more limited set of extracellular matrix proteins (1 ‑ 3). This may suggest a specialized role in microfibril biology. Functionally, the MAGP-2 RGD motif binds to the integrin alpha v beta 3, allowing cell attachment to microfibrils (4). MAGP‑2 is thought to facilitate microfibril assembly and induce elastin fiber formation (1 ‑ 3, 5). Through its binding to EGF repeats, MAGP‑2 mediates the metalloproteinase-dependent release of Jagged1, and the metalloproteinase-independent release of Notch extracellular domains (6, 7). In endothelial cells, MAGP-2 promotes angiogenic sprouting by inhibiting Notch signaling, by binding to integrin alpha v beta 3 (which then enhances VEGF signaling), and/or enhancing the endothelial cell response to FGF basic and EGF (8, 9). In ovarian cancer, MAGP-2 expression is associated with cancer cell survival, increased microvessel density, and poor prognosis (10). Skin MAGP-2 expression is increased in human scleroderma and the tight skin (TSK) mouse and may contribute to abnormal collagen accumulation in these conditions by increasing the half-life of type I collagen (11, 12). Mutations within the MAGP-2-binding region of human Fibrillin-1 can be found in Marfan syndrome (5).

References

Gibson, M.A. et al. (1996) J. Biol. Chem. 271:1096.
Hanssen, E. et al. (2004) J. Biol. Chem. 279:29185.
Segade, F. et al. (2002) J. Biol. Chem. 277:11050.
Gibson, M.A. et al. (1999) J. Biol. Chem. 274:13060.
Lemaire, R. et al. (2007) J. Biol. Chem. 282:800.
Nehring, L.C. et al. (2005) J. Biol. Chem. 280:20349.
Miyamoto, A. et al. (2006) J. Biol. Chem. 281:10089.
Albig, A.R. et al. (2008) Microvasc. Res. 76:7.
Albig, A.R. et al. (2007) Angiogenesis 10:197.
Mok, S.C. et al. (2009) Cancer Cell 16:521.
Lemaire, R. et al. (2005) Arthritis Rheum. 52:1812.
Ito, S. et al. (2006) Am.J. Respir. Cell Mol. Biol. 34:688.

Long Name

Microfibril-associated Glycoprotein 2

Entrez Gene IDs

8076 (Human); 50530 (Mouse); 362429 (Rat)

Alternate Names

MAGP2; MAGP-2; MAGP2MFAP-5; MFAP5; Microfibril-associated glycoprotein 2; microfibrillar associated protein 5; microfibrillar-associated protein 5; MP25; MP25microfibril-associated glycoprotein-2