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Recombinant Mouse MFG-E8 Protein

Catalog #: 2805-MF
36 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2805-MF-050/CF

With Carrier

Catalog # Availability Size / Price Qty
2805-MF-050
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Citations (36)
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Recombinant Mouse MFG-E8 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 10-50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse MFG-E8 protein
Ala23-Cys463, with a C-terminal 6-His tag
Accession #
P21956
N-terminal Sequence
Analysis
Ala23
Predicted Molecular Mass
49.8 kDa
SDS-PAGE
64-72 kDa, reducing conditions

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2805-MF (with carrier)

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2805-MF/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2805-MF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2805-MF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MFG-E8

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C‑terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue‑protective role impairs anti‑tumor immunity and chemotherapy‑induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).

References
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Long Name
Milk Fat Globule EGF Factor 8
Entrez Gene IDs
4240 (Human); 17304 (Mouse)
Alternate Names
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47

Citations for Recombinant Mouse MFG-E8 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. MFG-E8 has guiding significance for the prognosis and treatment of sepsis
    Authors: W Wu, J Wang, J Chen, J Lu, Y Lao, K Huang, J Lin
    Scientific Reports, 2022-12-03;12(1):20916.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production
    Authors: D Pan, W Wu, G Zuo, X Xie, H Li, X Ren, C Kong, W Zhou, Z Zhang, M Waterfall, S Chen
    Cellular Signalling, 2022-07-26;98(0):110419.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. MFG-E8 promotes tendon-bone healing by regualting macrophage efferocytosis and M2 polarization after anterior cruciate ligament reconstruction
    Authors: R Geng, Y Lin, M Ji, Q Chang, Z Li, L Xu, W Zhang, J Lu
    Journal of Orthopaedic Translation, 2022-05-11;34(0):11-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. MFG-E8 Reduces Aortic Intimal Proliferation in a Murine Model of Transplant Vasculopathy
    Authors: B Brilland, P Laplante, P Thebault, K Geoffroy, MJ Brissette, M Latour, M Chassé, S Qi, MJ Hébert, H Cardinal, JF Cailhier
    International Journal of Molecular Sciences, 2022-04-07;23(8):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation
    Authors: M Ikemoto-Ue, H Zhou, T Kurosawa, Y Yoshimoto, M Toyoda, N Kanazawa, T Nakazawa, M Morita, K Tsuchida, A Uezumi
    Aging Cell, 2021-12-24;0(0):e13536.
    Species: Mouse
    Sample Types: Protein
    Applications: Reference Standard
  6. Acute RhoA/Rho Kinase Inhibition Is Sufficient to Restore Phagocytic Capacity to Retinal Pigment Epithelium Lacking the Engulfment Receptor MerTK
    Authors: Y Mao, SC Finnemann
    Cells, 2021-07-29;10(8):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  7. MFG-E8 Regulates Vascular Smooth Muscle Cell Migration Through Dose-Dependent Mediation of Actin Polymerization
    Authors: HY Chiang, PH Chu, SC Chen, TH Lee
    Journal of the American Heart Association, 2021-05-27;10(11):e020870.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway
    Authors: Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai
    Cell Death & Disease, 2021-05-25;12(6):533.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice
    Authors: HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan
    Cells, 2021-03-25;10(4):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  10. Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3
    Authors: NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann
    Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Propofol suppresses microglial phagocytosis through the downregulation of MFG-E8
    Authors: X Cai, Y Li, X Zheng, R Hu, Y Li, L Xiao, Z Wang
    Journal of Neuroinflammation, 2021-01-09;18(1):18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells
    Authors: HY Chiang, PH Chu, TH Lee
    J. Biomed. Sci., 2019-08-30;26(1):61.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  13. Milk Fat Globule-Epidermal Growth Factor-Factor 8 Reverses Lipopolysaccharide-Induced Microglial Oxidative Stress
    Authors: J Li, X Xu, X Cai, Y Weng, Y Wang, Q Shen, X Shi
    Oxid Med Cell Longev, 2019-03-13;2019(0):2601394.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  14. Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia
    Authors: J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin
    Cell Rep, 2018-08-21;24(8):1939-1948.e4.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  15. Expression and Characterisation of ?v?5 Integrin on Intestinal Macrophages
    Authors: AK Kumawat, C Yu, EA Mann, A Schridde, SC Finnemann, AM Mowat
    Eur. J. Immunol., 2018-05-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Inflammatory bone loss associated with MFG-E8 deficiency is rescued by teriparatide
    Authors: MN Michalski, AL Seydel, EM Siismets, LE Zweifler, AJ Koh, BP Sinder, JI Aguirre, K Atabai, H Roca, LK McCauley
    FASEB J., 2018-02-22;0(0):fj201701238R.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions
    Authors: Y Wang, G Luo, J Chen, R Jiang, J Zhu, N Hu, W Huang, G Cheng, M Jia, B Su, N Zhang, T Cui
    Sci Rep, 2017-02-14;7(0):42642.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Mfge8 regulates enterocyte lipid storage by promoting enterocyte triglyceride hydrolase activity
    JCI Insight, 2016-11-03;1(18):e87418.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. A novel affinity-based method for the isolation of highly purified extracellular vesicles
    Sci Rep, 2016-09-23;6(0):33935.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  20. Milk fat globule epidermal growth factor-8 limits tissue damage through inflammasome modulation during renal injury
    Authors: Marie-Joëlle Brissette
    J Leukoc Biol, 2016-06-03;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  21. Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes
    J Immunol, 2016-05-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  22. MFG-E8 drives melanoma growth by stimulating mesenchymal stromal cell-induced angiogenesis and M2 polarization of tumor-associated macrophages
    Cancer Res, 2016-05-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Milk fat globule-EGF factor VIII attenuates CNS injury by promoting neural stem cell proliferation and migration after cerebral ischemia.
    Authors: Cheyuo C, Aziz M, Yang W, Jacob A, Zhou M, Wang P
    PLoS ONE, 2015-04-13;10(4):e0122833.
    Species: Mouse, Rat
    Sample Types: Whole Cells, Whole Organism
    Applications: Bioassay, In Vivo
  24. Protective effect of MFG-E8 after cutaneous ischemia-reperfusion injury.
    Authors: Uchiyama A, Yamada K, Perera B, Ogino S, Yokoyama Y, Takeuchi Y, Ishikawa O, Motegi S
    J Invest Dermatol, 2014-12-10;135(4):1157-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  25. Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis.
    Authors: Soki F, Koh A, Jones J, Kim Y, Dai J, Keller E, Pienta K, Atabai K, Roca H, McCauley L
    J Biol Chem, 2014-07-08;289(35):24560-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8.
    Authors: Abe T, Shin J, Hosur K, Udey M, Chavakis T, Hajishengallis G
    J Immunol, 2014-06-23;193(3):1383-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay, In Vivo
  27. Tumour necrosis factor alpha-induced neuronal loss is mediated by microglial phagocytosis.
    Authors: Neniskyte U, Vilalta A, Brown G
    FEBS Lett, 2014-06-06;588(17):2952-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. MFG-E8 regulates angiogenesis in cutaneous wound healing.
    Authors: Uchiyama A, Yamada K, Ogino S, Yokoyama Y, Takeuchi Y, Udey M, Ishikawa O, Motegi S
    Am J Pathol, 2014-05-15;184(7):1981-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
    Authors: Wang X, Rodda L, Bannard O, Cyster J
    J Immunol, 2014-04-16;192(10):4601-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid beta toxicity.
    J Neuroinflammation, 2012-06-28;9(0):148.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2.
    Authors: Aziz M, Matsuda A, Yang WL, Jacob A, Wang P
    J. Immunol., 2012-05-25;189(1):393-402.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  32. MFG-E8 released by apoptotic endothelial cells triggers anti-inflammatory macrophage reprogramming.
    Authors: Brissette MJ, Lepage S, Lamonde AS, Sirois I, Groleau J, Laurin LP, Cailhier JF
    PLoS ONE, 2012-04-30;7(4):e36368.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Pre-treatment of recombinant mouse MFG-E8 downregulates LPS-induced TNF-alpha production in macrophages via STAT3-mediated SOCS3 activation.
    Authors: Aziz M, Jacob A, Matsuda A, Wu R, Zhou M, Dong W, Yang WL, Wang P
    PLoS ONE, 2011-11-15;6(11):e27685.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  34. Protective effect of milk fat globule-epidermal growth factor-factor VIII after renal ischemia-reperfusion injury in mice.
    Authors: Matsuda A, Wu R, Jacob A, Komura H, Zhou M, Wang Z, Aziz MM, Wang P
    Crit. Care Med., 2011-09-01;39(9):2039-47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  35. Milk fat globule epidermal growth factor-8 blockade triggers tumor destruction through coordinated cell-autonomous and immune-mediated mechanisms.
    Authors: Jinushi M, Sato M, Kanamoto A, Itoh A, Nagai S, Koyasu S, Dranoff G, Tahara H
    J. Exp. Med., 2009-05-11;206(6):1317-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. MFG-E8 regulates microglial phagocytosis of apoptotic neurons.
    Authors: Fuller AD, Van Eldik LJ
    J Neuroimmune Pharmacol, 2008-08-01;3(4):246-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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