Recombinant Mouse NKG2A (CHO-expressed) Fc Protein, CF

Catalog #: 9198-NK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9198-NK-050

Recombinant Mouse NKG2A (CHO-expressed) Fc Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse NKG2A (CHO-expressed) Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse NKG2A/CD159a Fc Chimera is coated at 0.5 µg/mL, recombinant human CD94 binds with a typical ED₅₀ of
0.1-0.6 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse NKG2A/CD159a protein

MDP	Mouse IgG_2A (Glu98-Lys330)	IEGR	Mouse NKG2A (Ala94-IIe244) Accession # Q9Z202
N-terminus			C-terminus

Accession #

Q9Z202

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

44 kDa

SDS-PAGE

58-67 kDa, reducing conditions

Product Datasheets

9198-NK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9198-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 μg/mL, recombinant human CD94 binds with a typical ED₅₀ of 0.1-0.6 μg/mL.

Reconstitution Calculator

Background: NKG2A/CD159a

NKG2A/CD159a is an approximately 40 kDa transmembrane C-type lectin superfamily protein that inhibits innate immune system activation (1). Mouse NKG2A consists of a 70 amino acid (aa) cytoplasmic domain with two ITIM inhibitory motifs, a 23 aa transmembrane segment, and a 151 aa extracellular domain (ECD) with one C-type lectin domain (2). Within the ECD, mouse NKG2A shares 41% and 71% aa sequence identity with human and rat NKG2A, respectively. Alternative splicing generates additional isoforms with a 17 aa deletion in the extracellular juxtamembrane region or a substitution of that region plus the transmembrane segment. NKG2A is expressed on a subset of NK cells and CD8⁺ T cells (2-6) where it forms a covalent heterodimer with CD94 (5, 7, 8). NKG2A-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1^b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (2, 7). Triggering the NKG2A-CD94 complex inhibits the cytolytic activity of NK and CD8⁺ T cells (2, 3, 5, 6, 9). This enables the innate immune system to detect cells that express host MHC-I molecules and to protect them from NK cell mediated lysis. This mechanism is subverted by human cytomegalovirus which encodes a peptide that is homologous to the HLA-E binding peptide (10). HCMV infected cells up-regulate both HLA-E and NKG2A expression and utilize this peptide to escape from immune clearance (3, 10). In contrast, vaccinia virus induces HLA-E down-regulation, thus permitting NK cell lysis of the virally infected cell (11).

References

Das, J. and S.I. Khakoo (2015) Immunol. Rev. 267:214.
Vance, R.E. et al. (1998) J. Exp. Med. 188:1841.
Saez-Borderias, A. et al. (2009) J. Immunol.182:829.
Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
Zhou, J. et al. (2008) J. Immunol. 180:25.
Braud, V.M. et al. (1998) Nature 391:795.
Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
Lee, N. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5199.
Tomasec, P. et al. (2000) Science 287:1031.
Brooks, C.R. et al. (2006) J. Immunol. 176:1141.

Long Name

Natural Killer G2A

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Alternate Names

CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B