Recombinant Mouse ROBO3 Fc Chimera Protein, CF

Catalog #: 3155-RB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3155-RB-050

Recombinant Mouse ROBO3 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ROBO3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Slit3 (Catalog # 9296-SL) is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse ROBO3 Fc Chimera that produces 50% of the optimal binding response is 0.1-0.7 μg/mL

Source

Mouse myeloma cell line, NS0-derived mouse ROBO3 protein

Mouse ROBO3 (Gly54-Ser545) Accession # Q9Z2I4	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9Z2I4

N-terminal Sequence
Analysis

Gly54

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

81 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Product Datasheets

3155-RB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3155-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Slit3 (Catalog # 9296-SL) is immobilized at 0.5 µg/mL, 100 µL/well, Recombinant Mouse ROBO3 Fc Chimera (Catalog # 3155-RB) binds with an ED₅₀ of 0.1-0.7 µg/mL.

Reconstitution Calculator

Background: ROBO3

Mouse ROBO3 (also named Rig-1) is a 200 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Mouse ROBO3 is a type I transmembrane glycoprotein that is synthesized as a 1366 amino acid (aa) precursor. It contains a 20 aa signal sequence, an 871 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 454 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 64 - 531) and three fibronectin (FN) type III domains (aa 555-863). The cytoplasmic region contains three of four possible 15-20 aa long CC (conserved cytoplasmic) motifs that are found in ROBO-1 (7, 8). Mouse ROBO3 has multiple isoforms. An alternate start site generates an 1366 aa (precursor) A isoform and a 1344 aa (precursor) B isoform. These two forms only differ in the first 53 and 31 amino acids of the precursor, respectively (9). There are reportedly nine splice variants in the mouse ROBO3 gene. Three result in soluble forms. Little information exists about the isoforms. Mouse ROBO3 ECD is 95%, 84% and 86% aa identical to the ROBO3 ECD in rat, human and canine, respectively. Normally, axons originating on one side of the spinal cord are inhibited from crossing to the other side by a SLIT2-ROBO1 interaction at the midline. ROBO3 is permissive for this event. It is unclear how this is accomplished. One possibility is that it binds directly to ROBO-1, blocking SLIT activation. A second possibility involves ROBO3 binding to SLIT2 in a nonproductive interaction. In human, only ROBO3 Form B is known to bind to SLIT2 (9-11).

References

Rajagopalan, S. et al. (2000) Neuron 28:767.
Guthrie, S. (2004) Curr. Biol. 14:R632.
Guthrie, S. (2001) Curr. Biol. 11:R300.
Seeger, M. et al. (1993) Neuron 10:409.
Yuan, S.-S. F. et al. (1999) Dev. Biol. 207:62.
SwissProt. Accession # Q9Z2I4.
Bashaw, G.J. et al. (2000) Cell 101:703.
Kidd, T. et al. (1998) Cell 92:205.
Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
Sabatier, C. et al. (2004) Cell 117:157.
Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.

Long Name

Roundabout Homolog 3

Entrez Gene IDs

64221 (Human); 19649 (Mouse)

Alternate Names

FLJ21044; HGPPS; HGPS; RBIG1horizontal gaze palsy with progressive scoliosis; retinoblastoma inhibiting gene 1; RIG1; ROBO3; roundabout (axon guidance receptor, Drosophila) homolog 3; roundabout homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); Roundabout-like protein 3