Recombinant Mouse SIGNR7/CD209g Protein, CF

Catalog #: 5198-SR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5198-SR-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SIGNR7/CD209g Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. When 5 x 10⁴ cells/well are added to rmSIGN-R7 coated plates (5 μg/mL, 100 μL/well), approximately 70%-90% will adhere after 1 hour at 37° C. Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse SIGNR7/CD209g protein
Arg71-Tyr255 with an N-terminal 6-His tag

Accession #

CAL15171

N-terminal Sequence
Analysis

6 His-Arg71

Predicted Molecular Mass

21.9 kDa

SDS-PAGE

29-30 kDa reducing conditions

Product Datasheets

5198-SR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5198-SR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SIGNR7/CD209g

SIGNR7 (dendritic cell-specific ICAM-3 grabbing nonintegrin related gene 7), also known as CD209g, belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens. SIGNR7 is one of several mouse proteins which are encoded by separate genes and exhibit structural and sequence homology to human DC-SIGN and DC-SIGNR (1 - 3). Mouse SIGNR7 is a type 2 membrane protein that consists of a 45 amino acid (aa) cytoplasmic domain, a 22 aa transmembrane segment, and a 188 aa extracellular domain with a juxtamembrane neck region and a single carbohydrate recognition domain (CRD). Within the CRD, mouse SIGNR7 shares 44% - 49% aa sequence identity with mouse SIGNR1, 2, 3, 4, and 5 and 81% with SIGNR8. It shares 44% - 54% aa sequence identity with the CRD of bovine SIGNR7, human DC-SIGN, human DC-SIGNR, and rat DC-SIGN. A SIGNR7 ortholog has been described in cow but not in human (4). SIGNR7 binds both fucose and mannose residues and shows a distinct binding preference for the 6’-sulfo-sialyl Lewis× carbohydrate (5). Its interaction with mannosylated BSA is disrupted at low pH (5).

References